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与细胞逆转录病毒样（VL30）元件相关的长末端重复序列的启动子和增强子活性。

Promoter and enhancer activities of long terminal repeats associated with cellular retrovirus-like (VL30) elements.

作者信息

Rotman G, Itin A, Keshet E

出版信息

Nucleic Acids Res. 1986 Jan 24;14(2):645-58. doi: 10.1093/nar/14.2.645.

DOI:10.1093/nar/14.2.645
PMID:3456150
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339455/
Abstract

LTR units associated with cellular retrovirus-like elements are abundantly present in chromosomal DNA of animal cells. We have analyzed the promoter and enhancer activities of diverse LTR units associated with different members of the murine retrovirus-like family known as VL30. We report here that the structurally heterogenous VL30 LTRs displayed highly variable promoter/enhancer activities. The most active VL30 LTR (designated VL3) promoted CAT activity to levels six-fold higher than the LTR of the strongly transforming retrovirus, MSV. This VL30 transcription unit, containing a unique U3 region, was further characterized by S1 nuclease mapping. VL3 LTR functioned as an enhancer in CAT constructs containing a SV40 promoter. In addition, a defined U3 segment was shown to augment expression of CAT in an orientation independent manner. VL3 LTR also served as an efficient promoter and enhancer in heterologous monkey cells. These results suggest that certain resident LTRs possess promoter and enhancer capacities greater than those possessed by LTRs of infectious retroviruses.

摘要

与细胞逆转录病毒样元件相关的长末端重复序列（LTR）大量存在于动物细胞的染色体DNA中。我们分析了与小鼠逆转录病毒样家族不同成员（称为VL30）相关的多种LTR的启动子和增强子活性。我们在此报告，结构异质的VL30 LTR显示出高度可变的启动子/增强子活性。最活跃的VL30 LTR（命名为VL3）将氯霉素乙酰转移酶（CAT）活性提高到比强转化逆转录病毒MSV的LTR高六倍的水平。这个包含独特U3区域的VL30转录单位通过S1核酸酶图谱进一步表征。VL3 LTR在含有SV40启动子的CAT构建体中起增强子作用。此外，一个确定的U3片段被证明以方向独立的方式增强CAT的表达。VL3 LTR在异源猴细胞中也作为有效的启动子和增强子。这些结果表明，某些常驻LTR具有比感染性逆转录病毒的LTR更大的启动子和增强子能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/d024d5cde94d/nar00271-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/2e3c2f900f17/nar00271-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/5911a822330b/nar00271-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/0d4999319a63/nar00271-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/3ce1a1886b0b/nar00271-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/d024d5cde94d/nar00271-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/2e3c2f900f17/nar00271-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/5911a822330b/nar00271-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/0d4999319a63/nar00271-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/3ce1a1886b0b/nar00271-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd26/339455/d024d5cde94d/nar00271-0044-a.jpg

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本文引用的文献

1
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
2
Homology between an endogenous viral LTR and sequences inserted in an activated cellular oncogene.内源性病毒长末端重复序列与插入激活的细胞癌基因中的序列之间的同源性。
Nature. 1983 Apr 7;302(5908):547-8. doi: 10.1038/302547a0.
3
Amounts, synthesis, and some properties of intracisternal A particle-related RNA in early mouse embryos.
缺氧诱导的大鼠VL30元件及其与含ras肉瘤病毒的关系。
J Virol. 1993 Nov;67(11):6857-62. doi: 10.1128/JVI.67.11.6857-6862.1993.
4
Inducible and cell type-specific expression of VL30 U3 subgroups correlate with their enhancer design.VL30 U3亚组的可诱导性和细胞类型特异性表达与其增强子设计相关。
J Virol. 1994 Jan;68(1):276-88. doi: 10.1128/JVI.68.1.276-288.1994.
5
An internal ribosomal entry signal in the rat VL30 region of the Harvey murine sarcoma virus leader and its use in dicistronic retroviral vectors.哈维鼠肉瘤病毒前导序列大鼠VL30区域中的内部核糖体进入信号及其在双顺反子逆转录病毒载体中的应用。
J Virol. 1995 Oct;69(10):6400-7. doi: 10.1128/JVI.69.10.6400-6407.1995.
6
Transient expression of a chloramphenicol acetyltransferase gene following transfection of Physarum polycephalum myxamoebae.多头绒泡菌变形虫转染后氯霉素乙酰转移酶基因的瞬时表达。
Curr Genet. 1988;13(1):71-4. doi: 10.1007/BF00365759.
7
Complete nucleotide sequence of a mouse VL30 retro-element.小鼠VL30逆转录元件的完整核苷酸序列。
Mol Cell Biol. 1988 Aug;8(8):2989-98. doi: 10.1128/mcb.8.8.2989-2998.1988.
8
Harvey sarcoma virus genome contains no extensive sequences unrelated to those of other retroviruses except ras.哈维肉瘤病毒基因组除了癌基因ras外，不包含与其他逆转录病毒无关的大量序列。
J Virol. 1988 Sep;62(9):3540-3. doi: 10.1128/JVI.62.9.3540-3543.1988.
9
Isolation and characterization of a family of rat endogenous retroviral sequences.大鼠内源性逆转录病毒序列家族的分离与鉴定
Virus Genes. 1989 Sep;3(1):69-83. doi: 10.1007/BF00301988.
10
Independent regulation of mouse VL30 retrotransposon expression in response to serum and oncogenic cell transformation.小鼠VL30逆转录转座子表达对血清和致癌细胞转化的独立调控。
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4
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Proc Natl Acad Sci U S A. 1983 May;80(10):2946-50. doi: 10.1073/pnas.80.10.2946.
5
Unusual long terminal repeat sequence of a retrovirus transmissible mouse (VL 30) genetic element: identification of functional domains.一种可传播的逆转录病毒小鼠（VL 30）遗传元件的异常长末端重复序列：功能域的鉴定
Nucleic Acids Res. 1984 Apr 25;12(8):3445-60. doi: 10.1093/nar/12.8.3445.
6
Early differential tissue expression of transposon-like repetitive DNA sequences of the mouse.小鼠转座子样重复DNA序列的早期差异组织表达
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5641-5. doi: 10.1073/pnas.80.18.5641.
7
Family of middle repetitive DNA sequences in the mouse genome with structural features of solitary retroviral long terminal repeats.小鼠基因组中具有单独逆转录病毒长末端重复序列结构特征的中等重复DNA序列家族。
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3327-30. doi: 10.1073/pnas.80.11.3327.
8
A common region for proviral DNA integration in MoMuLV-induced rat thymic lymphomas.莫洛尼鼠白血病病毒（MoMuLV）诱导的大鼠胸腺淋巴瘤中前病毒DNA整合的常见区域。
Nature. 1983;302(5907):445-9. doi: 10.1038/302445a0.
9
Polyadenylylated RNA complementary to a mouse retrovirus-like multigene family is rapidly and specifically induced by epidermal growth factor stimulation of quiescent cells.与小鼠逆转录病毒样多基因家族互补的聚腺苷酸化RNA在静止细胞受到表皮生长因子刺激后被快速且特异性地诱导产生。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7317-21. doi: 10.1073/pnas.79.23.7317.
10
The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.劳氏肉瘤病毒长末端重复序列通过DNA介导转染导入多种真核细胞时是一个强启动子。
Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777-81. doi: 10.1073/pnas.79.22.6777.