Animal Physiology and Neurobiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.
Plant Physiology and Biotechnology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.
Molecules. 2019 Oct 20;24(20):3775. doi: 10.3390/molecules24203775.
Great effort is put into seeking a new and effective strategies to control insect pests. One of them is to combine natural products with chemical insecticides to increase their effectiveness. In the study presented, menthol which is an essential oil component was evaluated on its ability to increase the efficiency of bendiocarb, carbamate insecticide. A multi-approach study was conducted using biochemical method (to measure acetylcholinesterase enzyme activity), electrophysiological technique (microelectrode recordings in DUM neurons in situ), and confocal microscopy (for calcium imaging). In the electrophysiological experiments, menthol caused hyperpolarization, which was blocked by an octopamine receptor antagonist (phentolamine) and an inhibitor of protein kinase A (H-89). It also raised the intracellular calcium level. The effect of bendiocarb was potentiated by menthol and this phenomenon was abolished by phentolamine and H-89 but not by protein kinase C inhibitor (bisindolylmaleimide IX). The results indicate that menthol increases carbamate insecticide efficiency by acting on octopamine receptors and triggering protein kinase A phosphorylation pathway.
人们投入大量精力来寻找新的、有效的控制害虫的策略。其中之一是将天然产物与化学杀虫剂结合使用，以提高它们的效果。在本研究中，评估了薄荷醇（一种精油成分）对提高氨基甲酸酯类杀虫剂丙硫克百威的效率的能力。采用生化方法（测量乙酰胆碱酯酶活性）、电生理学技术（在原位 DUM 神经元中进行微电极记录）和共聚焦显微镜（钙成像）进行了多方法研究。在电生理学实验中，薄荷醇引起超极化，该超极化被章鱼胺受体拮抗剂（苯肾上腺素）和蛋白激酶 A 抑制剂（H-89）阻断，同时还提高了细胞内钙水平。薄荷醇增强了丙硫克百威的作用，这种现象被苯肾上腺素和 H-89 消除，但被蛋白激酶 C 抑制剂（双吲哚马来酰亚胺 IX）消除。结果表明，薄荷醇通过作用于章鱼胺受体并触发蛋白激酶 A 磷酸化途径来提高氨基甲酸酯类杀虫剂的效率。
