Lu Ting, Zheng Yingchun, Yang Haiying, Wu Buling, Xiong Jun, Huang Cheng, Pan Yuhua, Li Meiyi, He Fei, Xiong Fu
School of Stomatology, Southern Medical University; Department of Endodontics, National Key Specialty of Endodontics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
School of Basic Medical Science, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Sep 30;39(9):1113-1117. doi: 10.12122/j.issn.1673-4254.2019.09.18.
To explore the histological structure of the deciduous teeth and the tooth germs of Tibetan miniature pigs for studies of dental tissue diseases and tooth regeneration.
The structure of the deciduous teeth of Tibetan miniature pigs was observed by X-ray. The ultrastructure of the enamel and dentin of deciduous teeth was characterized by scanning electron microscopy. The jaws and teeth were three-dimensionally reconstructed using Mimics software based on Micro-CT scanning of the deciduous teeth. Image J software was used to calculate the gray value and the mineralization density of the deciduous teeth. Hisotological structure of the tooth germ and the pulp tissue of Tibetan miniature pigs was observed using HE staining.
The deciduous teeth of Tibetan miniature pigs were composed of enamel, dentin and medullary pulp tissue. The permanent tooth germ were formed during the deciduous dentition. The enamel and dentin ultrastructure of deciduous teeth were consistent with that of human deciduous teeth. The enamel and dentin mineralization densities were 2.47±0.09 g/cm and 1.72±0.07 g/cm, respectively. The pathological structures of tooth germ and pulp tissue were similar to those of human teeth, and the pulp tissue of the deciduous teeth was in an undifferentiated state.
The deciduous teeth of Tibetan miniature pig have similar anatomy, ultrastructure and histopathological structure to human teeth and can serve as a good animal model for studying human dental tissue diseases and the mechanisms of tooth regeneration.
探究藏猪乳牙及牙胚的组织结构，为牙体组织疾病及牙齿再生研究提供依据。
采用X线观察藏猪乳牙的结构；利用扫描电子显微镜观察乳牙釉质和牙本质的超微结构；基于乳牙的Micro-CT扫描图像，使用Mimics软件对颌骨和牙齿进行三维重建；运用Image J软件计算乳牙的灰度值和矿化密度；采用HE染色观察藏猪牙胚和牙髓组织的组织学结构。
藏猪乳牙由釉质、牙本质和髓腔牙髓组织构成，在乳牙列期恒牙胚开始形成。乳牙釉质和牙本质的超微结构与人乳牙的一致，釉质和牙本质的矿化密度分别为2.47±0.09 g/cm和1.72±0.07 g/cm。牙胚和牙髓组织的病理结构与人牙相似，乳牙牙髓组织处于未分化状态。
藏猪乳牙在解剖结构、超微结构及组织病理学结构方面与人牙相似，可作为研究人类牙体组织疾病及牙齿再生机制的良好动物模型。
