Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, China.
Outpatient Department of Oral and Maxillofacial Surgery, Capital Medical University School of Stomatology, Beijing, China.
J Mol Histol. 2018 Oct;49(5):545-553. doi: 10.1007/s10735-018-9792-1. Epub 2018 Aug 11.
Bone morphogenetic proteins (BMPs) play important roles in tooth development. However, their expression has not been studied in miniature pigs, which have many anatomical similarities in oral and maxillofacial region compared to human. This study investigated BMP2/4/7 expression patterns during deciduous molar development in miniature pigs on embryonic days (E) 40, 50, and 60. The mandibles were fixed, decalcified, and embedded before sectioning. H&E staining, immunohistochemistry, in situ hybridization using specific radionuclide-labeled cRNA probes, and real-time PCR were used to detect the BMP expression patterns during morphogenesis of the third deciduous molar. H&E staining showed that for the deciduous third molar, E40 represented the cap stage, E50 represented the early bell stage, and E60 represented the late bell stage or secretory stage. BMP2 was expressed in both the enamel organ and in the dental mesenchyme on E40 and E50 and was expressed mainly in pre-odontoblasts on E60. BMP7 expression was similar to BMP2 expression, but BMP7 was also expressed in the inner enamel epithelium on E60. On E40, BMP4 was expressed mainly in the epithelium, with some weak expression in the mesenchyme. On E50, BMP4 expression was stronger in the mesenchyme but weaker in the epithelium. On E60, BMP4 was expressed mainly in the mesenchyme. These data indicated that BMP2/4/7 showed differential spatial and temporal expression during the morphogenesis and odontogenesis of deciduous molars, suggesting that these molecules were associated with tooth morphogenesis and cell differentiation.
骨形态发生蛋白（BMPs）在牙齿发育中发挥重要作用。然而，它们在小型猪中的表达尚未得到研究，与人类相比，小型猪在口腔和颌面区域具有许多解剖学上的相似之处。本研究在胚胎第 40、50 和 60 天（E）研究了小型猪乳磨牙发育过程中 BMP2/4/7 的表达模式。将下颌骨固定、脱钙、包埋后进行切片。使用特定放射性核素标记的 cRNA 探针进行 H&E 染色、免疫组织化学、原位杂交和实时 PCR，以检测 BMP 在第三乳磨牙形态发生过程中的表达模式。H&E 染色显示，对于乳磨牙，E40 代表帽状期，E50 代表早期钟状期，E60 代表晚期钟状期或分泌期。BMP2 在 E40 和 E50 时既在釉质器官中表达，也在牙间质中表达，在 E60 时主要在前期成牙本质细胞中表达。BMP7 的表达与 BMP2 的表达相似，但 BMP7 也在 E60 时的内釉上皮中表达。在 E40 时，BMP4 主要在上皮中表达，间质中有一些弱表达。在 E50 时，BMP4 在间质中的表达更强，但在上皮中的表达较弱。在 E60 时，BMP4 主要在间质中表达。这些数据表明，BMP2/4/7 在乳磨牙形态发生和牙发生过程中表现出不同的时空表达，表明这些分子与牙齿形态发生和细胞分化有关。
