Ulrich F
Immunology. 1982 Jun;46(2):369-80.
Rat spleen cells were mitogenically stimulated with concanavalin A (Con A) or sodium periodate and cultured for 14-20 hr with trypsin. When these trypsin-activated cells were co-cultured with fresh mitogenically stimulated cells, [3H]-thymidine incorporation into the fresh cells was suppressed. Artifactual and trivial effects of trypsin (e.g. increased release of cold thymidine, decreased cell viability, and a change in thymidine transport) could not account for the suppressor effect. Suppressor activity was not affected by removing B cells or macrophages before trypsin activation nor by treating the trypsinized cells with mitomycin C. Suppressor activity could only be generated when the spleen cells were stimulated with Con A or periodate during culture with trypsin, and supernates from the trypsin-activated cells did not have suppressor activity. The physiological significance of the results and possible mechanisms of action of the suppressor cells are discussed.
用刀豆球蛋白A（Con A）或高碘酸钠对大鼠脾细胞进行有丝分裂原刺激，并用胰蛋白酶培养14 - 20小时。当这些经胰蛋白酶激活的细胞与新鲜的经有丝分裂原刺激的细胞共培养时，新鲜细胞中[³H] - 胸腺嘧啶核苷的掺入受到抑制。胰蛋白酶的人为和微不足道的影响（例如冷胸腺嘧啶核苷释放增加、细胞活力降低以及胸腺嘧啶核苷转运变化）不能解释这种抑制作用。在胰蛋白酶激活前去除B细胞或巨噬细胞，或用丝裂霉素C处理胰蛋白酶消化的细胞，均不影响抑制活性。只有当脾细胞在胰蛋白酶培养期间用Con A或高碘酸钠刺激时，才能产生抑制活性，且胰蛋白酶激活细胞的上清液没有抑制活性。讨论了这些结果的生理意义以及抑制细胞可能的作用机制。
