McDaniel M C, Tucker M A, Johnson D A
Inflammation. 1983 Dec;7(4):339-45. doi: 10.1007/BF00916298.
Trypsin, when added to a bioassay for tumoricidal macrophages, produced killing of tumor cells. Trypsin cleaved fetal calf serum proteins to produce a protein fragment that activated macrophages to lyse tumor cells. Diisopropyl fluorophosphate-inhibited trypsin did not produce tumoricidal macrophages either by direct action on the macrophage or by action on serum proteins. The macrophage activation factors produced from serum proteins were fractionated into molecular weight ranges of 150,000, 68,000, and 30,000-5000. The effects of neutral proteinases and proteinase inhibitors on the ability of macrophages to lyse tumor cells is discussed.
将胰蛋白酶添加到用于检测杀肿瘤巨噬细胞的生物测定中时，会导致肿瘤细胞死亡。胰蛋白酶裂解胎牛血清蛋白，产生一种能激活巨噬细胞以裂解肿瘤细胞的蛋白片段。经二异丙基氟磷酸抑制的胰蛋白酶，无论是直接作用于巨噬细胞还是作用于血清蛋白，都不会产生杀肿瘤巨噬细胞。从血清蛋白产生的巨噬细胞激活因子被分离成分子量范围为150,000、68,000和30,000 - 5000的组分。本文讨论了中性蛋白酶和蛋白酶抑制剂对巨噬细胞裂解肿瘤细胞能力的影响。
