Área de Genómica y Biotecnología, IFAPA, Alameda del Obispo, Córdoba, Spain.
Department of Genetics - ETSIAM, University of Córdoba, Campus de Rabanales, Córdoba, Spain.
PLoS One. 2019 Oct 23;14(10):e0224212. doi: 10.1371/journal.pone.0224212. eCollection 2019.
Chickpea production may be seriously threatened by Fusarium wilt, a disease caused by the soil-borne fungus Fusarium oxysporum f. sp. ciceris. F. oxysporum race 5 is the most important race in the Mediterranean basin. Recently, the region responsible for resistance race 5 has been delimited within a region on chromosome 2 that spans 820 kb. To gain a better understanding of this genomic region, we used a transcriptomic approach based on quantitative real-time PCR to analyze the expression profiles of 22 selected candidate genes. We used a pair of near-isogenic lines (NILs) differing in their sensitivity to Fusarium race 5 (resistant vs susceptible) to monitor the transcriptional changes over a time-course experiment (24, 48, and 72 hours post inoculation, hpi). Qualitative differences occurred during the timing of regulation. A cluster of 12 genes were induced by the resistant NIL at 24 hpi, whereas a second cluster contained 9 genes induced by the susceptible NIL at 48 hpi. Their possible functions in the molecular defence of chickpea is discussed. Our study provides new insight into the molecular defence against Fusarium race 5 and demonstrates that development of NILs is a rich resource to facilitate the detection of candidate genes. The new genes regulated here may be useful against other Fusarium races.
鹰嘴豆的生产可能会受到枯萎病的严重威胁，这种疾病是由土壤传播的真菌尖孢镰刀菌引起的。尖孢镰刀菌 5 号是在地中海盆地最重要的一个菌株。最近，对抵抗 5 号菌株的区域进行了限定，它位于横跨 820kb 的 2 号染色体区域内。为了更好地理解这个基因组区域，我们使用了基于定量实时 PCR 的转录组学方法来分析 22 个选定候选基因的表达谱。我们使用一对近等基因系（NILs），它们在对尖孢镰刀菌 5 号菌株的敏感性方面存在差异（抗性与敏感），以监测随时间变化的转录变化（接种后 24、48 和 72 小时，hpi）。在调控时间上出现了定性差异。一组 12 个基因在 24 hpi 时被抗性 NIL 诱导，而第二组包含 9 个基因在 48 hpi 时被敏感 NIL 诱导。讨论了它们在鹰嘴豆分子防御中的可能功能。我们的研究为对抗尖孢镰刀菌 5 号菌株的分子防御提供了新的见解，并表明开发 NILs 是一个丰富的资源，可以促进候选基因的检测。这里调控的新基因可能对其他尖孢镰刀菌菌株有用。
