Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.
Department of Chemical Sciences, Afe Babalola University Ado-Ekiti, P.M.B, Ado-Ekiti, 5454, Nigeria.
Biol Trace Elem Res. 2020 Jul;196(1):153-167. doi: 10.1007/s12011-019-01907-z. Epub 2019 Oct 28.
Accumulation of lanthanum oxide nanoparticles (LaONPs) in the femur bone of rats after 30 days of oral administration was explored. Also, the influence of LaONPs on macro and trace elements in the rats' femur bone was assessed. Inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical spectrometry (ICP OES) were used for total element determination in the bone after decomposition while laser ablation-ICP-MS (LA-ICP-MS) was used to investigate element distribution (bio-imaging) in the bone. Some differences in element concentrations in the bone between the rats treated with LaONPs at 1.0 mg kg (T1), 10.0 mg kg (T2), and 100 mg kg (T3) body weight (bw) and the control rats (CTR) were observed. More differences were observed in the bone of rat treated with 10.0 mg kg LaONPs bw. However, the highest change observed was for Mg, which concentration ranged from 5230 ± 12 μg kg for the CTR group to 4130 ± 138 μg kg for the T3 group. Minor changes were observed for Ba, Ca, Cr, Cu, Fe, Mg, Na, Pb, Sr, and Zn between CTR and animals treated with LaONPs at the different levels of concentration. It was possible to observe from LA-ICP-MS analysis that LaONPs were accumulated only on the surface of the bone, not deeper than about 5 μm. LA-ICP-MS allowed also to investigate the distribution of La and the other elements in a cross section of the femur bone head, where higher amounts of the elements are present at the external part of the bone. Therefore, it was demonstrated that LaONPs are incorporated on the surface of the bone and it has a small influence on some of the other elements evaluated.
研究了经口给予大鼠 30 天后氧化镧纳米颗粒（LaONPs）在股骨中的积累情况。此外，还评估了 LaONPs 对大鼠股骨中宏量和微量元素的影响。采用电感耦合等离子体质谱法（ICP-MS）和电感耦合等离子体光学发射光谱法（ICP-OES）对骨中总元素进行测定，采用激光烧蚀-电感耦合等离子体质谱法（LA-ICP-MS）对骨中元素分布（生物成像）进行研究。观察到经 1.0mg/kg（T1）、10.0mg/kg（T2）和 100.0mg/kg（T3）体重的 LaONPs 处理的大鼠与对照组（CTR）的骨中元素浓度存在一些差异。在经 10.0mg/kg LaONPs 体重处理的大鼠骨中观察到更多差异。然而，观察到的最大变化是 Mg，其浓度范围从对照组的 5230±12μg/kg 到 T3 组的 4130±138μg/kg。在 CTR 和用不同浓度 LaONPs 处理的动物之间，Ba、Ca、Cr、Cu、Fe、Mg、Na、Pb、Sr 和 Zn 的浓度变化较小。从 LA-ICP-MS 分析可以看出，LaONPs 仅在骨表面积累，深度不超过约 5μm。LA-ICP-MS 还允许研究股骨头部横截面中 La 和其他元素的分布，其中骨的外部存在更多的元素。因此，证明 LaONPs 被整合到骨表面，并且对评估的一些其他元素的影响较小。
