Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Int J Mol Sci. 2023 Feb 14;24(4):3842. doi: 10.3390/ijms24043842.
We examined the photodynamic activation of Curcumin under blue light in glioblastoma T98G cells. The therapeutic effect of Curcumin, in both the absence and presence of blue light, was measured by the MTT assay and apoptosis progression using flow cytometry. Fluorescence imaging was carried out to evaluate Curcumin uptake. Photodynamic activation of Curcumin (10 µM), in the presence of blue light, enhanced its cytotoxic effect, resulting in the activation of ROS-dependent apoptotic pathways in T98G cells. The gene expression studies showed the expression of matrixes metalloproteinase 2 (MMP2) and 9 (MMP9) decrease with Curcumin (10 µM) under blue light exposure, indicating possible proteolytic mechanisms. Moreover, the cytometric appearance displayed that the expressions of NF-κB and Nrf2 were found to be increased upon exposure to blue light, which revealed a significant induction of expression of nuclear factor as a result of blue-light-induced oxidative stress and cell death. These data further demonstrate that Curcumin exhibited a photodynamic effect via induction of ROS-mediated apoptosis in the presence of blue light. Our results suggest that the application of blue light enhances the therapeutic efficacy of Curcumin in glioblastoma because of the phototherapeutic effect.
我们研究了姜黄素在神经胶质瘤 T98G 细胞中在蓝光下的光动力激活作用。通过 MTT 测定和流式细胞术检测细胞凋亡进程，检测姜黄素在无蓝光和有蓝光条件下的治疗效果。荧光成像用于评估姜黄素的摄取。在蓝光存在的条件下，姜黄素（10 μM）的光动力激活增强了其细胞毒性作用，导致 T98G 细胞中 ROS 依赖性凋亡途径的激活。基因表达研究表明，基质金属蛋白酶 2 (MMP2)和 9 (MMP9)的表达在蓝光暴露下随着姜黄素（10 μM）的作用而减少，表明可能存在蛋白水解机制。此外，细胞计外观显示，NF-κB 和 Nrf2 的表达在暴露于蓝光时增加，这表明由于蓝光诱导的氧化应激和细胞死亡，核因子的表达显著诱导。这些数据进一步表明，姜黄素在蓝光存在下通过诱导 ROS 介导的细胞凋亡表现出光动力效应。我们的研究结果表明，由于光疗作用，蓝光的应用增强了姜黄素在神经胶质瘤中的治疗效果。
