莫沸气对实验性心肌缺血氧化应激/抗氧化平衡的影响。
The Effect of Mofettes on Oxidative Stress/Antioxidant Balance in Experimental Myocardial Ischemia.
机构信息
Department of Medical Rehabilitation, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Pathophysiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
出版信息
In Vivo. 2019 Nov-Dec;33(6):1911-1920. doi: 10.21873/invivo.11685.
BACKGROUND/AIM: Natural mofettes are gases resulting from post-volcanic emanations. This study aimed to examine the effect of mofette therapy on plasma oxidative stress and antioxidant parameters in rats after experimental induction of myocardial ischemia, as well as on structural changes in myocardial tissue.
MATERIALS AND METHODS
White Wistar-Bratislava rats were divided into three groups. In groups 2 and 3, myocardial ischemia was induced by isoproterenol. Rats in group 3 were additionally exposed to high levels mofettes. Oxidative stress and antioxidant parameters were determined in plasma. The structural changes of the myocardium were observed in paraffin embedded slices contrasted using Goldner's trichrome staining.
RESULTS
A statistically significant change in serum oxidative stress biomarkers, including nitric oxide, malondialdehyde, total oxidant status, as well as in the tested antioxidant molecules and total antioxidant capacity were observed in group 3 compared to group 2. Also, rats of group 3 showed an obvious improvement in inflammatory infiltration and repair of necrotic areas through collagen proliferation (proliferation of fibrous connective tissue) compared to group 2.
CONCLUSION
Mofette had a beneficial effect on the balance between oxidative stress and antioxidant status following experimentally induced myocardial ischemia.
背景/目的：天然沼气是火山喷发后释放的气体。本研究旨在探讨沼气疗法对实验诱导心肌缺血后大鼠血浆氧化应激和抗氧化参数的影响，以及对心肌组织结构变化的影响。
材料和方法
将白色 Wistar-Bratislava 大鼠分为三组。在第 2 组和第 3 组中，通过异丙肾上腺素诱导心肌缺血。第 3 组大鼠还接受了高水平的沼气暴露。在血浆中测定氧化应激和抗氧化参数。使用 Goldner 三色染色对比石蜡包埋切片观察心肌组织的结构变化。
结果
与第 2 组相比，第 3 组的血清氧化应激生物标志物（包括一氧化氮、丙二醛、总氧化剂状态）以及测试的抗氧化分子和总抗氧化能力发生了统计学上的显著变化。此外，与第 2 组相比，第 3 组的大鼠通过胶原增殖（纤维结缔组织增殖）显示出对炎症浸润和坏死区域修复的明显改善。
结论
沼气对实验诱导的心肌缺血后氧化应激和抗氧化状态之间的平衡具有有益的影响。
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