噬菌体vB_SauS_SA2的分离与鉴定
Isolation and characterization of the bacteriophage vB_SauS_SA2.
作者信息
Wang Jia, Zhao Feiyang, Sun Huzhi, Wang Qian, Zhang Can, Liu Wenhua, Zou Ling, Pan Qiang, Ren Huiying
机构信息
Qingdao Agricultural University, College of Veterinary Medicine, Shandong 266109, China.
Qingdao Phagepharm Bio-tech Co, Ltd, Shandong 266109, China.
出版信息
AIMS Microbiol. 2019 Sep 27;5(3):285-307. doi: 10.3934/microbiol.2019.3.285. eCollection 2019.
A novel bacteriophage vB_SauS_SA2 (hereafter designated SA2) that infects was isolated. At a multiplicity of infection (MOI) of 0.1, phage SA2 had a latent period of about 10 min with a burst size of 293 PFUs/infected cell (PFU, plaque forming unit). Phage SA2 had a double-stranded DNA genome with a length of 89,055 bp and a G + C content of 31.9%. The genome contained 130 open reading frames (ORFs), 28 of which had assigned functions, and 18 were unique. One tRNA gene ( ) was discovered, and no virulence genes were identified. Its genome showed very low similarity with phage genomes deposited in public databases (75% nucleotide identity and 7% query coverage). The unique characteristics of phage SA2 led to the proposal of a new genus named 'SA2likevirus'.
分离出一种新型噬菌体vB_SauS_SA2（以下简称SA2），它能感染……。在感染复数（MOI）为0.1时，噬菌体SA2的潜伏期约为10分钟，裂解量为293个噬菌斑形成单位/感染细胞（PFU，噬菌斑形成单位）。噬菌体SA2具有双链DNA基因组，长度为89,055 bp，G + C含量为31.9%。该基因组包含130个开放阅读框（ORF），其中28个具有已确定的功能，18个是独特的。发现了一个tRNA基因（……），未鉴定出毒力基因。其基因组与公共数据库中存储的噬菌体基因组显示出非常低的相似性（核苷酸同一性为75%，查询覆盖率为7%）。噬菌体SA2的独特特征导致提出了一个名为“SA2样病毒”的新属。
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