Chakrabarti Srijita, Goyary Danswrang, Karmakar Sanjeev, Chattopadhyay Pronobesh
Defence Research Laboratory, Tezpur, Assam, India.
Toxicol Ind Health. 2019 Sep;35(9):577-592. doi: 10.1177/0748233719879611.
Health hazards of titanium dioxide nanoparticles (TiO-NPs) have raised severe concerns because of the paucity of information regarding the toxic effects among the population. In the present research, the and cytotoxic potential of TiO-NPs were evaluated using flow cytometric techniques. Further, and genotoxic endpoints were estimated by means of comet, micronucleus (MN), and chromosomal aberration (CA) assays. analysis was performed at the concentration range of 10-100 µg/mL using murine RAW 264.7 cells. experiments were conducted on Albino mice (M/F) by exposing them to 200 and 500 mg/kg TiO-NPs for 90 days. Decreased percentage of cell viability with higher doses of TiO-NPs was evident in both and flow cytometric analysis. Further, an impaired cell cycle (G/G, S, and G/M) was reflected in the present investigation following the exposure to TiO-NPs. Increased comet scores such as tail length, % DNA in tail, tail moment, and olive moment were also observed with the higher doses of TiO-NPs and comet assays. Finally, the MN and CA assays revealed the formation of MN and chromosomal breakage following the exposure to TiO-NPs.
由于人群中关于二氧化钛纳米颗粒（TiO-NPs）毒性影响的信息匮乏，其健康危害引发了严重关注。在本研究中，使用流式细胞术评估了TiO-NPs的细胞毒性潜力。此外，通过彗星试验、微核（MN）试验和染色体畸变（CA）试验估计了遗传毒性终点。使用小鼠RAW 264.7细胞在10-100 µg/mL的浓度范围内进行了分析。通过将白化小鼠（雄性/雌性）暴露于200和500 mg/kg的TiO-NPs 90天进行了体内实验。在流式细胞术分析中，较高剂量的TiO-NPs均明显降低了细胞活力百分比。此外，本研究中暴露于TiO-NPs后反映出细胞周期（G0/G1、S和G2/M）受损。在较高剂量的TiO-NPs彗星试验中也观察到彗星评分增加，如尾长、尾中DNA百分比、尾矩和橄榄尾矩。最后，体内微核试验和染色体畸变试验显示暴露于TiO-NPs后形成了微核和染色体断裂。
