Olumayede Emmanuel Gbenga, Oguntimehin Ilemobayo, Babalola Bolanle, Ojiodu Chukuwebe C, Akinyeye Richard O, Sodipe Grace Olubunmi, Uche Joseph, Ojo Ayomipo
Department of Industrial Chemistry, Federal University, Oye, Ekiti, Nigeria.
Department of Chemical Sciences, Ondo State University of Science and Technology, Okitipupa, Ondo State, Nigeria.
MethodsX. 2019 Aug 23;6:1944-1949. doi: 10.1016/j.mex.2019.07.027. eCollection 2019.
This study was piloted to evaluate bioaccessibility of particulate-bound trace elements using synthetic epithelia lung fluid; in which dipalmitoylphophatidylcholine was substituted with locus bean gum (LBSFL). The resulting data reveal that no significant change in physicochemical characteristics of the stimulated lung fluid compare with similar synthetic fluids; pH value of 7.3, density (0.998gcm), conductivity (13.9 mS m), surface viscosity (1.136 × 10 pas) and surface tension (50.6 mN m). To prove the potential applicability of the fluid in bioaccessibility test, we compared bioaccessibility of particulates-bound trace elements using this fluid with those of stimulated epithelial lung fluid. Bioaccessibility were relatively low values (<30%) in locus bean substituted lung fluid and stimulated epithelial lung fluid. Specifically, As and Cd had significantly higher bioaccessibility values in locus bean substituted lung fluid than stimulated epithelial lung fluid. The data demonstrate that fluid formulated and used in this study can provide a suitable means of evaluate bioaccessibility of trace elements-bound to airborne particulates. •The fluid was used for assessing bioaccessibility of particulate matters-bound trace elements•The formulated fluid can be applied to study in toxicity assessment•The data can be used for inter-laboratory comparison of bioaccessibility of particulate -bound trace element and could stimulate environmental concerns on the impacts of airborne particulates.
本研究旨在使用合成上皮肺液评估颗粒结合微量元素的生物可及性；其中用刺槐豆胶替代了二棕榈酰磷脂酰胆碱（LBSFL）。所得数据表明，与类似的合成液相比，刺激肺液的物理化学特性没有显著变化；pH值为7.3，密度（0.998g/cm³），电导率（13.9 mS/m），表面粘度（1.136×10⁻³ Pa·s）和表面张力（50.6 mN/m）。为了证明该液体在生物可及性测试中的潜在适用性，我们将使用这种液体的颗粒结合微量元素的生物可及性与刺激的上皮肺液的生物可及性进行了比较。在刺槐豆替代肺液和刺激的上皮肺液中，生物可及性相对较低（<30%）。具体而言，在刺槐豆替代肺液中，砷和镉的生物可及性值明显高于刺激的上皮肺液。数据表明，本研究中配制和使用的液体可以提供一种评估与空气传播颗粒结合的微量元素生物可及性的合适方法。•该液体用于评估颗粒物结合微量元素的生物可及性•配制的液体可用于毒性评估研究•该数据可用于颗粒结合微量元素生物可及性的实验室间比较，并可能引发对空气传播颗粒影响的环境关注。
