Pereira Suiane S C, Araujo Geisoellen F, de Queiroz Lucas N, Câmara Priscilla R, Pascoal Vinícius D B, Azevedo Rebeca S, Robbs Bruno K
Postgraduate Program in Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Brazil.
Postgraduate Program in Applied Science for Health Products, Faculty of Pharmacy, Fluminense Federal University, Niteroi, Brazil.
MethodsX. 2019 Sep 17;6:2156-2165. doi: 10.1016/j.mex.2019.09.004. eCollection 2019.
Periodontal disease is one of the most common causes of tooth loss in the world. Ligature-induced is the most used method to study periodontitis. Here, we describe a alternative, easy and accessible experimental technique of ligation in mice. Twenty C57BL/6 female mice were divided in two groups, control and ligation. Ligature group (n = 10) was immobilized in a well described stabilization board and ligature was performed at the first molar using a new procedure here described in detail. Eight weeks later animals were euthanized, and periodontitis hallmarks were evaluated. Ligatures remained attached to the teeth in all animal during the hole experiment. The procedure induced a temporary loss of weight but no causalities or tooth loss. The animals affected by ligation in their molar teeth presented all periodontitis hallmarks, including alveolar bone loss, gingival retraction and inflammatory infiltrate in the studied region both macro and microscopically. The alternative method is low cost, easily reproducible, and induces all periodontitis hallmarks that are sustained until 8 weeks after placement. •Ligature-induced periodontitis in mouse is a powerful tool of research.•Methods describing the procedure in literature are difficult to reproduce.•A alternative stabilization and ligation procedure in mice is completely described here.
牙周病是全球牙齿缺失最常见的原因之一。结扎诱导法是研究牙周炎最常用的方法。在此，我们描述一种在小鼠中进行结扎的替代方法，该方法简便且易于操作。将20只C57BL/6雌性小鼠分为两组，即对照组和结扎组。结扎组（n = 10）被固定在一个详细描述的稳定板上，并使用此处详细描述的新方法在第一磨牙处进行结扎。八周后对动物实施安乐死，并评估牙周炎的特征。在整个实验过程中，结扎线均附着在所有动物的牙齿上。该操作导致动物体重暂时减轻，但无死亡或牙齿脱落情况。磨牙进行结扎的动物在宏观和微观上均呈现出所有牙周炎特征，包括牙槽骨丧失、牙龈退缩以及研究区域的炎性浸润。该替代方法成本低、易于重复，且能诱导出可持续至结扎后8周的所有牙周炎特征。•小鼠结扎诱导性牙周炎是一种强大的研究工具。•文献中描述该操作的方法难以重复。•本文完整描述了一种小鼠的替代稳定和结扎操作方法。
