Department of Mechanical Engineering, Islamic Azad University, Khomeinishahr Branch, Khomeinishahr 84175-119, Iran.
Department of Mechanical Engineering, Islamic Azad University, Khomeinishahr Branch, Khomeinishahr 84175-119, Iran.
Comput Methods Programs Biomed. 2020 Mar;185:105147. doi: 10.1016/j.cmpb.2019.105147. Epub 2019 Oct 21.
The presence of circulating tumor cells in the patient's blood is a clinical factor that indicates the initiation of metastasis.
In this paper, a microfluidic device is designed to separate, enumerate, and to diagnose cancer cells from the patient's blood sample. Here, we present a new microfluidic device that is capable of separating circulating tumor cells based on their physical properties by using electrophoresis and dielectrophoresis forces within an electrical field. Cells are modeled as spherical particles, each size as a representor of different cells.
The distance between cells has been increased, and by using a novel method of rolling up the convergent and divergent channels, the primary distance of elements, which was 50 µm, reached 142-178.5. Cells with different diameters sense a different force, and therefore, cancer cells can be separating from other cells in the blood, such as white blood cells. To make the current device, practical for clinical studies, a cell enumerator is designed for downstream of cell separator. In the cell enumerator, a change in impedance occurs when a cell passes through the sensor.
By measuring the electrical current between two electrodes of the cell enumerator sensor, the exact count of cells can be obtained.
患者血液中循环肿瘤细胞的存在是预示转移开始的临床因素。
本文设计了一种微流控装置，用于从患者的血液样本中分离、计数和诊断癌细胞。在这里，我们提出了一种新的微流控装置，该装置能够基于癌细胞的物理性质，通过在电场中利用电泳和介电泳力来分离循环肿瘤细胞。细胞被建模为球形颗粒，每个大小代表不同的细胞。
细胞之间的距离已经增加，并且通过使用新颖的方法卷起收敛和发散通道，将元件的原始距离从 50µm 增加到 142-178.5µm。具有不同直径的细胞感受到不同的力，因此，癌细胞可以与血液中的其他细胞（如白细胞）分离。为了使当前的设备更适用于临床研究，设计了细胞分离器的下游细胞计数器。在细胞计数器中，当细胞通过传感器时，阻抗会发生变化。
通过测量细胞计数器传感器两个电极之间的电流，可以获得细胞的确切计数。
