Institute of Animal Nutrition, Northeast Agricultural University, Harbin, 150030, PR China.
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, 150030, PR China.
Ecotoxicol Environ Saf. 2020 Jan 15;187:109846. doi: 10.1016/j.ecoenv.2019.109846. Epub 2019 Oct 31.
At present, the public is paying more attention to the adverse effects of pesticides on human and animal health and the environment. Glyphosate is a broad-spectrum pesticide that is widely used in agricultural production. In this manuscript, the effects of diets containing glyphosate on intestinal morphology, intestinal immune factors, intestinal antioxidant capacity and the mRNA expression associated with the Nrf2 signaling pathway were investigated in weaned piglets. Twenty-eight healthy female hybrid weaned piglets (Duroc × Landrace × Yorkshire) were randomly selected with an average weight of 12.24 ± 0.61 kg. Weaned piglets were randomly assigned into 4 treatment groups and fed a basal diet supplemented with 0, 10, 20, and 40 mg/kg glyphosate for a 35-day feeding trial. We found that glyphosate had no effect on intestinal morphology. In the duodenum, glyphosate increased the activities of CAT and SOD (linear, P < 0.05) and increased the levels of MDA (linear and quadratic, P < 0.05). In the duodenum, glyphosate remarkably increased the relative mRNA expression levels of Nrf2 (linear and quadratic, P < 0.05) and NQO1 (linear and quadratic, P < 0.05) and reduced the relative mRNA expression levels of GPx1, HO-1 and GCLM (linear and quadratic, P < 0.05). In the jejunum, glyphosate remarkably increased the relative mRNA expression levels of Nrf2 (linear and quadratic, P < 0.05) and decreased the relative mRNA expression levels of GCLM (linear and quadratic, P < 0.05). Glyphosate increased the mRNA expression levels of IL-6 in the duodenum (linear and quadratic, P < 0.05) and the mRNA expression levels of IL-6 in the jejunum (linear, P < 0.05). Glyphosate increased the mRNA expression of NF-κB in the jejunum (linear, P = 0.05). Additionally, the results demonstrated that glyphosate linearly decreased the ZO-1 mRNA expression levels in the jejunum and the mRNA expression of claudin-1 in the duodenum (P < 0.05). In the duodenum, glyphosate increased the protein expression levels of Nrf2 (linear, P = 0.025). Overall, glyphosate exposure may result in oxidative stress in the intestines of piglets, which can be alleviated by enhancing the activities of antioxidant enzymes and self-detoxification.
目前，公众越来越关注农药对人类健康和环境的不良影响。草甘膦是一种广泛使用的农业生产中的广谱农药。在本手稿中，研究了含有草甘膦的饮食对断奶仔猪肠道形态、肠道免疫因子、肠道抗氧化能力以及与 Nrf2 信号通路相关的 mRNA 表达的影响。选择 28 头健康雌性杂交断奶仔猪（杜洛克×长白×约克夏），平均体重 12.24±0.61kg，随机分为 4 个处理组，分别饲喂基础日粮，基础日粮中添加 0、10、20 和 40mg/kg 草甘膦，进行 35 天的饲养试验。结果表明，草甘膦对肠道形态没有影响。在十二指肠中，草甘膦增加了 CAT 和 SOD 的活性（线性，P<0.05）并增加了 MDA 的水平（线性和二次，P<0.05）。在十二指肠中，草甘膦显著增加了 Nrf2 的相对 mRNA 表达水平（线性和二次，P<0.05）和 NQO1 的相对 mRNA 表达水平（线性和二次，P<0.05），并降低了 GPx1、HO-1 和 GCLM 的相对 mRNA 表达水平（线性和二次，P<0.05）。在空肠中，草甘膦显著增加了 Nrf2 的相对 mRNA 表达水平（线性和二次，P<0.05），并降低了 GCLM 的相对 mRNA 表达水平（线性和二次，P<0.05）。草甘膦增加了十二指肠中 IL-6 的 mRNA 表达水平（线性和二次，P<0.05）和空肠中 IL-6 的 mRNA 表达水平（线性，P<0.05）。草甘膦增加了空肠中 NF-κB 的 mRNA 表达（线性，P=0.05）。此外，结果表明，草甘膦线性降低了空肠中 ZO-1 的 mRNA 表达水平和十二指肠中 Claudin-1 的 mRNA 表达水平（P<0.05）。在十二指肠中，草甘膦增加了 Nrf2 的蛋白表达水平（线性，P=0.025）。总的来说，草甘膦暴露可能导致仔猪肠道氧化应激，通过增强抗氧化酶活性和解毒能力可以缓解这种应激。
