Northway M G, Scobey M W, Geisinger K R
Department of Medicine, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103.
Cancer. 1988 Nov 1;62(9):1962-9. doi: 10.1002/1097-0142(19881101)62:9<1962::aid-cncr2820620916>3.0.co;2-t.
Female Wistar rats were treated with single exposure irradiation to 2 cm of distal colon to cause radiation proctitis. All animals were evaluated by examination, colonoscopy and histologic evaluation for changes post-irradiation. Exposures of 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5 and 30 Gy caused dose-related clinical and histologic changes peaking at 7 to 15 days post-exposure. Rats treated with 20 Gy were colonoscoped and biopsied daily and showed sequential post-irradiation endoscopic changes ranging from mucosal edema and mild inflammatory changes to erosion and ulcers. Histologically, crypt abscess and mural wall necrosis similar to changes found in the human rectum after radiotherapy were noted. Treatment with nonsteroidal anti-inflammatory agents, (aspirin, indomethacin, piroxicam), misoprostol (a prostaglandin E1 analogue), or sucralfate (an anti-ulcer agent) did not ameliorate nor exacerbate radiation proctitis in rats exposed to 22.5 Gy. We conclude from these data that the female Wistar rat is a good model for studying radiation proctitis because endoscopic, histologic, and clinical changes seen post-exposure closely resemble those found in man.
将雌性Wistar大鼠的远端结肠2厘米处单次暴露于辐射下，以引发放射性直肠炎。通过检查、结肠镜检查和组织学评估对所有动物照射后的变化进行评估。10、12.5、15、17.5、20、22.5、25、27.5和30 Gy的照射剂量引起了与剂量相关的临床和组织学变化，在照射后7至15天达到峰值。接受20 Gy照射的大鼠每天进行结肠镜检查和活检，结果显示照射后内镜变化呈连续性，从粘膜水肿和轻度炎症变化到糜烂和溃疡。组织学上，观察到隐窝脓肿和肠壁坏死，类似于人类放疗后直肠的变化。用非甾体抗炎药（阿司匹林、吲哚美辛、吡罗昔康）、米索前列醇（一种前列腺素E1类似物）或硫糖铝（一种抗溃疡药）治疗，并未改善或加重接受22.5 Gy照射大鼠的放射性直肠炎。从这些数据我们得出结论，雌性Wistar大鼠是研究放射性直肠炎的良好模型，因为照射后观察到的内镜、组织学和临床变化与人类的变化非常相似。
