Yang Jun-Li, Yang Yun-Han, Xun Yu-Peng, Wei Ke-Ke, Gu Jie, Chen Mei, Yang Li-Juan
School of Chemistry & Environment, Key Laboratory of Intelligent Supramolecular Chemistry at the University of Yunnan Province, National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials, Yunnan Minzu University, Kunming 650500, P. R. China.
ACS Omega. 2019 Oct 18;4(18):17903-17909. doi: 10.1021/acsomega.9b02951. eCollection 2019 Oct 29.
A novel fluorescent probe, amino-pillar[5]arene (APA), was prepared via a green, effective, and convenient synthetic method, which was characterized by nuclear magnetic resonance (NMR), infrared (IR), and high-resolution mass spectrometry. The fluorescence sensing behavior of the APA probe toward 22 metal ions in aqueous solutions were studied by fluorescence spectroscopy. The results showed that APA could be used as a selective fluorescent probe for the specificity detection of Au ions. Moreover, the detection characteristics were investigated by fluorescence spectral titration, pH effect, fluorescence competitive experiments, Job's plot analysis, H NMR, and IR. The results indicated that detection of Au ions by the APA probe could be achieved in the range of pH 1-13.5 and that other coexisting metal ions did not cause any marked interference. The titration analysis results indicated that the fluorescence intensity decreased as the concentration of Au ions increased, with an excellent correlation ( = 0.9942). The detection limit was as low as 7.59 × 10 mol·L, and the binding ratio of the APA probe with Au ions was 2:1. Therefore, the APA probe has potential applications for detecting Au ions in the environment and in living organisms.
通过一种绿色、高效且简便的合成方法制备了一种新型荧光探针氨基柱[5]芳烃（APA），并通过核磁共振（NMR）、红外（IR）和高分辨率质谱对其进行了表征。采用荧光光谱法研究了APA探针在水溶液中对22种金属离子的荧光传感行为。结果表明，APA可作为一种选择性荧光探针用于特异性检测金离子。此外，通过荧光光谱滴定、pH效应、荧光竞争实验、Job曲线分析、1H NMR和IR对检测特性进行了研究。结果表明，在pH 1 - 13.5范围内，APA探针均可实现对金离子的检测，且其他共存金属离子不会产生明显干扰。滴定分析结果表明，随着金离子浓度的增加，荧光强度降低，具有良好的相关性（r = 0.9942）。检测限低至7.59×10-8 mol·L-1，APA探针与金离子的结合比为2:1。因此，APA探针在环境和生物体内检测金离子方面具有潜在应用价值。
