Department of Orthopedic Surgery, Peking Union Medical College Hospital, Beijing, China.
Department of Orthopaedic Surgery, West China Hospital, Sichuan University, Chengdu, China.
Horm Metab Res. 2019 Nov;51(11):735-740. doi: 10.1055/a-1012-8571. Epub 2019 Nov 4.
Osteoarthritis (OA) is a degenerative chronic disease affecting the whole joint structures. With the increment in life expectancy and aging population, OA has become one of the largest socioeconomic burdens, associated with pain and loss of joint function. However, early laboratory tests of OA are still lacking. Therefore, new diagnostic tests for this disease are urgently needed. In this study, to gain an insight into the pathogenesis and the potential biomarkers of OA, we implemented a comparative urine proteomics study on OA patients and health people using iTRAQ-based mass spectrometry technology. Western blotting was used to validate the relative changes in urine protein levels for four of the identified proteins. We constructed a comprehensive urine proteome profile of the OA patients and identified 102 proteins differently changed in abundance. Forty-six proteins were upregulated and 56 proteins were significantly downregulated in OA patients. Furthermore, the proteins, COL-4, MMP9, adiponectin, and BBOX1 were validated through Western blots, which can serve as valuable candidate biomarkers and help to illustrate the pathogenesis of OA. These findings may provide clues for promising biomarkers for the early diagnosis and also offer a theoretical basis for the early treatment of OA.
骨关节炎（OA）是一种影响整个关节结构的退行性慢性疾病。随着预期寿命的增加和人口老龄化，OA 已成为最大的社会经济负担之一，与疼痛和关节功能丧失有关。然而，OA 的早期实验室检测仍然缺乏。因此，迫切需要针对这种疾病的新诊断测试。在这项研究中，为了深入了解 OA 的发病机制和潜在的生物标志物，我们使用基于 iTRAQ 的质谱技术对 OA 患者和健康人进行了比较性尿液蛋白质组学研究。Western blot 用于验证鉴定出的 4 种蛋白质的尿液蛋白水平的相对变化。我们构建了 OA 患者的综合尿液蛋白质组图谱，并鉴定出 102 种丰度差异变化的蛋白质。46 种蛋白质在 OA 患者中上调，56 种蛋白质显著下调。此外，通过 Western blot 验证了 COL-4、MMP9、脂联素和 BBOX1 这 4 种蛋白质，它们可以作为有价值的候选生物标志物，有助于阐明 OA 的发病机制。这些发现可能为 OA 的早期诊断提供有希望的生物标志物，并为 OA 的早期治疗提供理论基础。
