来自……的麦芽糖酶和蔗糖酶抑制成分的表征

Characterization of Maltase and Sucrase Inhibitory Constituents from .

作者信息

Li Wen-Tai, Chuang Yu-Hsuan, Hsieh Jung-Feng

机构信息

National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 11221, Taiwan.

Department of Food Science, Fu Jen Catholic University, Taipei 242, Taiwan.

出版信息

Foods. 2019 Nov 2;8(11):540. doi: 10.3390/foods8110540.

The inhibitory properties of epicatechin-(4,8)-epicatechingallate (B2-3'gallate), epicatechin gallate (ECG), and epicatechin (EC) isolated from toward maltase and sucrase were investigated. The half-maximal inhibitory concentration (IC) values for maltase were as follows: B2-3'--gallate (1.73 ± 1.37 μM), ECG (3.64 ± 2.99 μM), and EC (6.25 ± 1.84 μM). Inhibition kinetic assays revealed the inhibition constants (i) of the mixed-competitive inhibitors of maltase, as follows: B2-3'--gallate (1.99 ± 0.02 μM), ECG (3.14 ± 0.04 μM), and EC (7.02 ± 0.26 μM). These compounds also showed a strong inhibitory activity toward sucrase, and the IC values of B2-3'--gallate, ECG, and EC were 6.91 ± 3.41, 18.27 ± 3.99, and 18.91 ± 3.66 μM, respectively. Inhibition kinetic assays revealed the inhibition constants (i) of the mixed-competitive inhibitors of sucrase as follows: B2-3'--gallate (6.05 ± 0.04 μM), ECG (8.58 ± 0.08 μM), and EC (13.72 ± 0.15 μM). Overall, these results suggest that B2-3'--gallate, ECG, and EC are potent maltase and sucrase inhibitors.

研究了从[未提及来源]中分离出的表儿茶素-(4,8)-表儿茶素没食子酸酯（B2-3'没食子酸酯）、表儿茶素没食子酸酯（ECG）和表儿茶素（EC）对麦芽糖酶和蔗糖酶的抑制特性。麦芽糖酶的半数最大抑制浓度（IC）值如下：B2-3'--没食子酸酯（1.73±1.37μM）、ECG（3.64±2.99μM）和EC（6.25±1.84μM）。抑制动力学分析揭示了麦芽糖酶混合竞争性抑制剂的抑制常数（Ki）如下：B2-3'--没食子酸酯（1.99±0.02μM）、ECG（3.14±0.04μM）和EC（7.02±0.26μM）。这些化合物对蔗糖酶也表现出较强的抑制活性，B2-3'--没食子酸酯、ECG和EC的IC值分别为6.91±3.41、18.27±3.99和18.91±3.66μM。抑制动力学分析揭示了蔗糖酶混合竞争性抑制剂的抑制常数（Ki）如下：B2-3'--没食子酸酯（6.05±0.04μM）、ECG（8.58±0.08μM）和EC（13.72±0.15μM）。总体而言，这些结果表明B2-3'--没食子酸酯、ECG和EC是有效的麦芽糖酶和蔗糖酶抑制剂。