College of Medicine, Chengdu University, Chengdu 610106, Sichuan, China.
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Chengdu 610106, Sichuan, China.
Molecules. 2020 Aug 12;25(16):3664. doi: 10.3390/molecules25163664.
The chemical components, as well as the antibacterial and antioxidant activities of the essential oil (EO) and crude extracts prepared from were investigated. The essential oil was separated by hydrodistillation, and gas chromatography-mass spectrometry (GC-MS) was used to identify its constituents. A total of twenty-seven compounds was identified from the EO, and its major components were 1-octanol (42.217%), geraniol (19.914%), and 6-methyl-5-hepten-2-ol (13.151%). Solvent extraction and fractionation were applied for preparing the ethanol extract (crude extract, CE), petroleum ether extract (PE), ethyl acetate extract (EE), -butanol extract (BE), and water extract (WE). The CE, EE and BE were abundant in phenols and flavonoids, and EE had the highest total phenol and total flavonoid contents. Gallic acid, ethyl gallate, rosavin and herbacetin were identified in the EE. The antibacterial activity results showed that the EO exhibited moderate inhibitory activity to the typical clinic bacteria, and EE exhibited the strongest antibacterial activity among the five extracts. For the compounds, ethyl gallate showed the strongest inhibitory activity to the test bacteria, and its minimum inhibitory concentration (MIC) value and minimum bactericidal concentration (MBC) value for all the tested bacteria was 0.24 mg/mL and 0.48 mg/mL, respectively. The results of antioxidant activity showed that both CE and EE exhibited strong antioxidant activities in the DPPH radical scavenging and Fe reducing power tests, however, EO showed relatively weaker antioxidant ability. Ethyl gallate and rosavin exhibited excellent activity in the DPPH radical scavenging assay, and their IC value was 5.3 µg/mL and 5.9 µg/mL, respectively. Rosavin showed better reduction power activity than the other three compounds. These results could provide more evidence for the traditional use of , and would be helpful for improving its application further.
对 中的精油（EO）和粗提取物的化学组成、抗菌和抗氧化活性进行了研究。通过水蒸馏法分离精油，并采用气相色谱-质谱联用（GC-MS）鉴定其成分。从 EO 中鉴定出 27 种化合物，其主要成分为 1-辛醇（42.217%）、香叶醇（19.914%）和 6-甲基-5-庚烯-2-醇（13.151%）。采用溶剂提取和分级法制备乙醇提取物（粗提取物，CE）、石油醚提取物（PE）、乙酸乙酯提取物（EE）、正丁醇提取物（BE）和水提取物（WE）。CE、EE 和 BE 富含酚类和类黄酮，EE 总酚和总类黄酮含量最高。EE 中鉴定出没食子酸、没食子酸乙酯、罗莎文和草丙酮。抗菌活性结果表明，EO 对典型临床细菌表现出中等抑制活性，而 EE 在五种提取物中表现出最强的抗菌活性。对于化合物，没食子酸乙酯对测试细菌表现出最强的抑制活性，其对所有测试细菌的最小抑菌浓度（MIC）值和最小杀菌浓度（MBC）值分别为 0.24 mg/mL 和 0.48 mg/mL。抗氧化活性结果表明，CE 和 EE 在 DPPH 自由基清除和 Fe 还原能力测试中均表现出较强的抗氧化活性，而 EO 表现出相对较弱的抗氧化能力。没食子酸乙酯和罗莎文在 DPPH 自由基清除试验中表现出优异的活性，其 IC 值分别为 5.3 µg/mL 和 5.9 µg/mL。罗莎文的还原能力活性优于其他三种化合物。这些结果可为 的传统用途提供更多证据，并有助于进一步改善其应用。
