Department of Gynaecology and Obstetrics, Jingmen No. 2 People's Hospital, Jingmen, Hubei, China.
Eur Rev Med Pharmacol Sci. 2019 Oct;23(20):8788-8794. doi: 10.26355/eurrev_201910_19273.
To explore the role of long noncoding ribonucleic acid (lncRNA) KCNQ1OT1 in the proliferation, apoptosis, and migration of ovarian cancer cells via Wnt/β-catenin.
Ovarian cancer A2780 cells were divided into three groups, namely control group, KCNQ1OT1 overexpression group, and KCNQ1OT1 knockdown group. Next, the effect of KCNQ1OT1 on the proliferation of ovarian cancer A2780 cells was detected by cell counting kit-8 (CCK-8) assay. Wound healing assay and transwell assay were carried out to determine the influence of KCNQ1OT1 on the migration ability of ovarian cancer A2780 cells. The role of KCNQ1OT1 in the cell cycle of ovarian cancer A2780 cells was detected via flow cytometry. The impact of KCNQ1OT1 on the expression level of β-catenin protein in ovarian cancer A2780 cells was determined through Western blotting and fluorescence immunoassay.
The proliferation rate of cells was overtly decreased in KCNQ1OT1 knockdown group but significantly increased in KCNQ1OT1 overexpression group. The results of both wound healing and transwell assays showed that the migration ability of cells was reduced in KCNQ1OT1 knockdown group but raised in KCNQ1OT1 overexpression group. According to flow cytometry, the cell cycle was clearly arrested in the G0/G1 phase in KCNQ1OT1 knockdown group. The results of Western blotting and fluorescence immunoassay revealed that compared with that in control group, the expression level of β-catenin protein evidently declined in KCNQ1OT1 knockdown group, but it was notably elevated in KCNQ1OT1 overexpression group.
Increased lncRNA KCNQ1OT1 in ovarian cancer cells promotes the expression of β-catenin, thereby facilitating the proliferation and migration of ovarian cancer cells.
探讨长链非编码 RNA（lncRNA）KCNQ1OT1 通过 Wnt/β-连环蛋白在卵巢癌细胞增殖、凋亡和迁移中的作用。
将卵巢癌细胞 A2780 分为对照组、KCNQ1OT1 过表达组和 KCNQ1OT1 敲低组。然后，通过细胞计数试剂盒-8（CCK-8）检测 KCNQ1OT1 对卵巢癌细胞 A2780 增殖的影响。采用划痕愈合实验和 Transwell 实验检测 KCNQ1OT1 对卵巢癌细胞 A2780 迁移能力的影响。通过流式细胞术检测 KCNQ1OT1 对卵巢癌细胞 A2780 细胞周期的作用。通过 Western blot 和荧光免疫法检测 KCNQ1OT1 对卵巢癌细胞 A2780 中β-连环蛋白蛋白表达水平的影响。
KCNQ1OT1 敲低组细胞增殖率明显降低，而过表达组细胞增殖率明显升高。划痕愈合和 Transwell 实验结果表明，KCNQ1OT1 敲低组细胞迁移能力降低，而过表达组细胞迁移能力增强。根据流式细胞术，KCNQ1OT1 敲低组细胞周期明显停滞在 G0/G1 期。Western blot 和荧光免疫法的结果显示，与对照组相比，KCNQ1OT1 敲低组β-连环蛋白蛋白表达水平明显下降，而过表达组β-连环蛋白蛋白表达水平明显升高。
卵巢癌细胞中 lncRNA KCNQ1OT1 的增加促进了β-连环蛋白的表达，从而促进了卵巢癌细胞的增殖和迁移。
