Yao Gang, Zhang Hui, Luo Guoyong, Wang Zuhua, Zhao Hai, Zhang Jian, Dong Yuanqiu, Gao Yuan, Wu Shuguang
Institute of Laboratory Animal Science, Guizhou University of Traditional Chinese Medicine, Huaxi University Town, Dongqing South Road, Guiyang, 550025, China.
College of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, China.
Arch Microbiol. 2021 May;203(4):1477-1488. doi: 10.1007/s00203-020-02144-y. Epub 2021 Jan 4.
The gut microbiota inhabits the animal intestinal tract, and dysbiosis of the gut microbiota may result in disease. Senecio scandens has pharmaceutical antibacterial activities and is regarded as a broad-spectrum antibiotic in traditional Chinese medicine. Extracts of S. scandens are reported to show strong antimicrobial activity, and quercetin significantly decreases some species in the caecal microflora. However, the bactericidal effects of the extracts on the gut microbiota remain obscure. Here, we supplied ethanol extract of S. scandens, which might possibly be used as an alternative for chemical antibiotics, to mice to investigate the state of the intestinal microbiota. Our studies included a control group, low-, moderate-, and high-dose ethanol extract groups, and cefixime capsule group. The ethanol extract groups did not present reduced diversity or differences in the gut microbiota balance. There were significant differences between the ethanol extract and cefixime capsule groups in terms of the gut microbiota. The control and ethanol extract groups contained similar bacteria, which suggested that the ethanol extract has no inhibitory effect on the gut microbiota in vivo. Bifidobacteriales and Lactobacillus acidophilus were significantly increased in the high-dose group. Both secretory immunoglobulin A and mucin 2 concentrations increased as the dose of ethanol extract increased. The functional prediction differences between the control and ethanol extract groups decreased with increasing extract doses, which indicated that the low-dose and high-dose extract treatments might regulate different pathways and functions of the gut microbiota. The results also highlighted the prevention of bacterial drug resistance in the ethanol extract groups.
肠道微生物群栖息于动物肠道内，肠道微生物群失调可能导致疾病。千里光具有药用抗菌活性，在传统中药中被视为一种广谱抗生素。据报道，千里光提取物具有很强的抗菌活性，槲皮素能显著减少盲肠微生物群中的一些物种。然而，该提取物对肠道微生物群的杀菌作用仍不清楚。在此，我们给小鼠投喂可能用作化学抗生素替代品的千里光乙醇提取物，以研究肠道微生物群的状态。我们的研究包括一个对照组、低剂量、中剂量和高剂量乙醇提取物组以及头孢克肟胶囊组。乙醇提取物组在肠道微生物群多样性或平衡方面没有呈现出降低或差异。乙醇提取物组和头孢克肟胶囊组在肠道微生物群方面存在显著差异。对照组和乙醇提取物组含有相似的细菌，这表明乙醇提取物在体内对肠道微生物群没有抑制作用。高剂量组中双歧杆菌目和嗜酸乳杆菌显著增加。随着乙醇提取物剂量的增加，分泌型免疫球蛋白A和黏蛋白2的浓度均升高。对照组和乙醇提取物组之间的功能预测差异随着提取物剂量的增加而减小，这表明低剂量和高剂量提取物处理可能调节肠道微生物群的不同途径和功能。结果还突出了乙醇提取物组对细菌耐药性的预防作用。
