Department of Neural and Behavioral Sciences, College of Medicine, Penn State University, Hershey, PA, USA.
Department of Pathology, College of Medicine, Penn State University, Hershey, PA, USA.
Nat Commun. 2019 Nov 7;10(1):5067. doi: 10.1038/s41467-019-13057-w.
Anatomical atlases in standard coordinates are necessary for the interpretation and integration of research findings in a common spatial context. However, the two most-used mouse brain atlases, the Franklin-Paxinos (FP) and the common coordinate framework (CCF) from the Allen Institute for Brain Science, have accumulated inconsistencies in anatomical delineations and nomenclature, creating confusion among neuroscientists. To overcome these issues, we adopt here the FP labels into the CCF to merge the labels in the single atlas framework. We use cell type-specific transgenic mice and an MRI atlas to adjust and further segment our labels. Moreover, detailed segmentations are added to the dorsal striatum using cortico-striatal connectivity data. Lastly, we digitize our anatomical labels based on the Allen ontology, create a web-interface for visualization, and provide tools for comprehensive comparisons between the CCF and FP labels. Our open-source labels signify a key step towards a unified mouse brain atlas.
标准坐标的解剖图谱对于在共同的空间背景下解释和整合研究结果是必要的。然而，两个最常用的小鼠脑图谱，即富兰克林-帕克斯诺斯（FP）图谱和艾伦脑科学研究所的共同坐标框架（CCF），在解剖划分和命名上积累了不一致之处，给神经科学家带来了困惑。为了解决这些问题，我们在这里采用 FP 标签到 CCF 中，将标签合并到单个图谱框架中。我们使用细胞类型特异性转基因小鼠和 MRI 图谱来调整和进一步分割我们的标签。此外，我们使用皮质-纹状体连接数据为背侧纹状体添加了详细的分割。最后，我们基于艾伦本体对我们的解剖标签进行数字化，创建了一个可视化的网络界面，并提供了用于 CCF 和 FP 标签之间全面比较的工具。我们的开源标签标志着迈向统一的小鼠脑图谱迈出了关键的一步。
