来自日本的高毒力进化枝2、核糖体分型019/序列型67菌株。
Hypervirulent clade 2, ribotype 019/sequence type 67 strain from Japan.
作者信息
Saito Ryoichi, Usui Yukino, Ayibieke Alafate, Nakajima Jun, Prah Isaac, Sonobe Kazunari, Aiso Yoshibumi, Ito Shiori, Itsui Yasuhiro, Hadano Yoshiro, Nukui Yoko, Koike Ryuji, Tohda Shuji
机构信息
1Department of Molecular Microbiology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
2Department of Infection Control and Prevention, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan.
出版信息
Gut Pathog. 2019 Nov 4;11:54. doi: 10.1186/s13099-019-0336-3. eCollection 2019.
BACKGROUND
ribotype (RT) 019/sequence type (ST) 67 strains belong to a hypervirulent lineage closely related to RT027/ST1; however, limited data are available for hypervirulent clade 2 lineages in Japan. Herein, we report the draft genome of a strain B18-123 belonging to clade 2, RT019/ST67 for the first time in Japan.
RESULTS
The pathogenicity locus carried by B18-123 (19.6 kb) showed higher homology (97.29% nucleotide identity) with strain R20291 (RT027/ST1) than the reference strain 630 (RT012/ST54), and B18-123 harbored 8-nucleotide substitutions in . However, it did not contain an 18-base pair (bp) deletion or a single-bp deletion at position 117 in , which was identified in the previous strain R20291. A cytotoxicity assay revealed similar cytotoxicity levels between strains B18-123 and ATCC BAA-1870 (RT027/ST1). The B18-123 strain was found to be susceptible to metronidazole and vancomycin.
CONCLUSION
Our findings contribute to the further understanding of the characteristics of hypervirulent clade 2 including RT019/ST67 lineages.
背景
核糖体分型（RT）019/序列型（ST）67菌株属于与RT027/ST1密切相关的高毒力谱系；然而，关于日本高毒力进化枝2谱系的数据有限。在此，我们首次报道了日本一株属于进化枝2、RT019/ST67的B18 - 123菌株的基因组草图。
结果
B18 - 123菌株携带的致病位点（19.6 kb）与R20291菌株（RT027/ST1）的同源性高于参考菌株630（RT012/ST54）（核苷酸同一性为97.29%），并且B18 - 123在[具体位置]有8个核苷酸替换。然而，它在[具体位置]没有18个碱基对（bp）的缺失或之前在R20291菌株中鉴定出的第117位的单碱基缺失。细胞毒性试验显示B18 - 123菌株和ATCC BAA - 1870（RT027/ST1）之间的细胞毒性水平相似。发现B18 - 123菌株对甲硝唑和万古霉素敏感。
结论
我们的研究结果有助于进一步了解包括RT019/ST67谱系在内的高毒力进化枝2的特征。
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