Li Bing, Chen Xueping, Wu Yanrong, Gu Aixia, Zhang Jingjing, Luo Shuangxia, Gao Xiurui, Zhao Jianjun, Pan Xiuqing, Shen Shuxing
1Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding, 071000 China.
2Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050051 China.
Hortic Res. 2019 Nov 1;6:118. doi: 10.1038/s41438-019-0201-z. eCollection 2019.
The naturally occurring mutant eggplant line 05ms was identified with reverse thermosensitive genic male sterility (rTGMS), but its temperature-responsive fertility mechanisms remain largely unknown. Here, we studied the flower morphology, anther cellular structure, and genome-wide gene expression of this rTGMS line. Candidate genes for thermosensitive male sterility during the microspore development of 05ms and the temperature-insensitive line S63 under low-temperature (LT) and high-temperature (HT) conditions were identified. Under LT, tapetum cells were vacuolated and had delayed disintegration in 05ms. RNA-seq analysis indicated that DEGs were enriched in the KEGG pathways 'plant hormone signal transduction', 'starch and sucrose metabolism', and 'phenylpropanoid biosynthesis'. We identified two genes, () and (), which could potentially regulate eggplant anther development and may be candidate genes for rTGMS. Finally, we propose a working model of anther abortion for rTGMS in eggplant. responds to LT stress and causes expression changes in genes related to anther development, such as , and the cellular structure of the tapetum becomes abnormal, causing male sterility. The findings of this study explain the underlying molecular mechanisms of male sterility in eggplant rTGMS lines.
天然存在的突变茄子品系05ms被鉴定为具有反向温敏核雄性不育（rTGMS），但其温度响应育性机制仍 largely未知。在此，我们研究了该rTGMS品系的花形态、花药细胞结构和全基因组基因表达。鉴定了05ms和温度不敏感品系S63在低温（LT）和高温（HT）条件下小孢子发育过程中温敏雄性不育的候选基因。在LT条件下，05ms的绒毡层细胞液泡化且解体延迟。RNA-seq分析表明，差异表达基因（DEGs）富集在KEGG途径“植物激素信号转导”、“淀粉和蔗糖代谢”以及“苯丙烷生物合成”中。我们鉴定了两个基因，（）和（），它们可能潜在地调节茄子花药发育，可能是rTGMS的候选基因。最后，我们提出了茄子rTGMS花药败育的工作模型。响应LT胁迫并导致与花药发育相关基因的表达变化，如，绒毡层的细胞结构变得异常，导致雄性不育。本研究结果解释了茄子rTGMS品系雄性不育的潜在分子机制。
