Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China.
College of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, China.
Food Chem. 2020 Mar 30;309:125779. doi: 10.1016/j.foodchem.2019.125779. Epub 2019 Oct 25.
Germinated edible seeds and sprouts are becoming increasingly common in the human diet because they are rich in bioactive compounds and antioxidants and are highly nutritious. In this study, the effects of NaCl stress and supplemental CaCl on carotenoid accumulation, antioxidant capacity and expression of key enzymes in yellow maize kernels were investigated. The results showed that the lutein and zeaxanthin contents increased with NaCl treatment, and further increased with supplemental CaCl. Additionally, germinated yellow maize kernels showed increased antioxidant capacity in response to NaCl and CaCl. The transcript levels of carotenogenic genes ZmPSY and ZmCYP97C were upregulated and the expression levels of ZmLCYB and ZmBCH1 were downregulated under NaCl stress. The expression of all key carotenogenic genes was upregulated by CaCl supplementation. These results suggested that NaCl and CaCl contribute to carotenoid accumulation via increased expression of related carotenogenic genes and increased antioxidant capacity in germinated yellow maize kernels.
发芽的可食用种子和芽菜在人类饮食中越来越常见，因为它们富含生物活性化合物和抗氧化剂，并且具有很高的营养价值。在这项研究中，研究了 NaCl 胁迫和补充 CaCl 对黄玉米籽粒类胡萝卜素积累、抗氧化能力和关键酶表达的影响。结果表明，叶黄素和玉米黄质含量随着 NaCl 处理的增加而增加，并且随着补充 CaCl 的增加而进一步增加。此外，发芽的黄玉米籽粒对 NaCl 和 CaCl 的响应表现出增强的抗氧化能力。在 NaCl 胁迫下，类胡萝卜素生物合成基因 ZmPSY 和 ZmCYP97C 的转录水平上调，而 ZmLCYB 和 ZmBCH1 的表达水平下调。CaCl 补充剂可上调所有关键类胡萝卜素生物合成基因的表达。这些结果表明，NaCl 和 CaCl 通过增加相关类胡萝卜素生物合成基因的表达和提高发芽黄玉米籽粒的抗氧化能力来促进类胡萝卜素的积累。
