测定体液中阿米洛利的简易高效液相色谱法。

Simple high-performance liquid chromatographic method for the measurement of amiloride in body fluids.

作者信息

Forrest G, McInnes G T, Fairhead A P, Thompson G G, Brodie M J

机构信息

University Department of Medicine, Gardiner Institute, Western Infirmary, Glasgow, Scotland, U.K.

出版信息

J Chromatogr. 1988 Jun 24;428(1):123-30. doi: 10.1016/s0378-4347(00)83896-7.

DOI:10.1016/s0378-4347(00)83896-7

PMID:3170664

Abstract

A simplified rapid high-performance liquid chromatographic (HPLC) procedure has been developed for the measurement of amiloride in plasma or urine. Solid-phase extraction columns provide quick, clean and simple sample preparation, allowing ten samples to be processed in less than 5 min. The HPLC system uses a standard reversed-phase (C18) column with detection by ultraviolet absorption at 365 nm. The assay has been used to define plasma amiloride concentration-time profiles and to quantitate urine amiloride recovery in healthy men after repeated administration at two doses.

摘要

已开发出一种简化的快速高效液相色谱（HPLC）方法，用于测定血浆或尿液中的阿米洛利。固相萃取柱可提供快速、清洁且简单的样品制备，能在不到5分钟的时间内处理10个样品。HPLC系统使用标准反相（C18）柱，通过365nm的紫外吸收进行检测。该测定法已用于确定健康男性在两种剂量重复给药后血浆中阿米洛利的浓度-时间曲线，并对尿液中阿米洛利的回收率进行定量。