Markesich D C, Graham D Y, Yoshimura H H
Inflammatory Bowel Disease Laboratory, Veterans Administration Medical Center, Houston, Texas 77030.
J Clin Microbiol. 1988 Aug;26(8):1600-3. doi: 10.1128/jcm.26.8.1600-1603.1988.
The efficiency of culture media was compared for the culture and subculture of very slowly growing acid-fast bacilli and spheroplast forms obtained from intestinal tissues of patients with Crohn's disease and ulcerative colitis and from controls without inflammatory bowel disease. Media were developed by modifying a nutrient broth medium based on veal infusion broth and yeast extract. We evaluated the effects of pH and the addition of Tween 80, Dubo oleic albumin complex, an extract from intestinal tissue from a patient with Crohn's disease, horse serum, sucrose, magnesium sulfate, ferrous ammonium sulfate, and sodium citrate. All media contained mycobactin J (2 micrograms/ml). We developed a medium (MG3) which was highly successful in promoting the growth of very fastidious organisms and promoted reversion of spheroplasts to acid-fast rods. MG3 contained veal infusion broth, 1% yeast extract, 10% horse serum, 0.3 M sucrose, 0.2% MgSO4, 0.1% ferrous ammonium sulfate, 0.1% sodium citrate, and 2 mg of mycobactin J per liter. We were able to obtain quantities of organisms sufficient for examination of the organisms by molecular techniques. Successful cultivation of all isolates and reversion of spheroplasts to acid-fast forms encourage further studies of the possibility of a complex association of mycobacteria and Crohn's disease.
比较了几种培养基对从克罗恩病和溃疡性结肠炎患者以及无炎症性肠病的对照者的肠道组织中获得的生长极其缓慢的抗酸杆菌和原生质球形式进行培养和传代培养的效率。通过基于小牛肉浸液肉汤和酵母提取物改良营养肉汤培养基来开发培养基。我们评估了pH值以及添加吐温80、杜博油酸白蛋白复合物、一名克罗恩病患者的肠道组织提取物、马血清、蔗糖、硫酸镁、硫酸亚铁铵和柠檬酸钠的影响。所有培养基均含有结核分枝杆菌生长素J（2微克/毫升）。我们开发了一种培养基（MG3），它在促进极难培养的微生物生长以及促进原生质球回复为抗酸杆菌方面非常成功。MG3含有小牛肉浸液肉汤、1%酵母提取物、10%马血清、0.3M蔗糖、0.2%硫酸镁、0.1%硫酸亚铁铵、0.1%柠檬酸钠以及每升2毫克结核分枝杆菌生长素J。我们能够获得足够数量的微生物，以便通过分子技术对其进行检测。所有分离株的成功培养以及原生质球回复为抗酸形式，促使人们进一步研究分枝杆菌与克罗恩病复杂关联的可能性。
