Victor R G, Bertocci L A, Pryor S L, Nunnally R L
Department of Internal Medicine, Harry S. Moss Heart Center, Dallas, Texas.
J Clin Invest. 1988 Oct;82(4):1301-5. doi: 10.1172/JCI113730.
We used phosphorus nuclear magnetic resonance spectroscopy (31P-NMR) to probe the cellular events in contracting muscle that initiate the reflex stimulation of sympathetic outflow during exercise. In conscious humans, we performed 31P-NMR on exercising forearm muscle and simultaneously recorded muscle sympathetic nerve activity (MSNA) with microelectrodes in the peroneal nerve to determine if the activation of MSNA is coupled to muscle pH, an index of glycolysis, or to the concentrations (II) of inorganic phosphate (Pi) and adenosine diphosphate (ADP) which are modulators of mitochondrial respiration. During both static and rhythmic handgrip, the onset of sympathetic activation in resting muscle coincided with the development of cellular acidification in active muscle. Furthermore, increases in MSNA were correlated closely with decreases in intracellular pH but dissociated from changes in phosphocreatine [( PCr]), [Pi], and [ADP]. The principal new conclusion is that activation of muscle sympathetic outflow during exercise in humans is coupled to the cellular accumulation of protons in contracting muscle.
我们利用磷核磁共振波谱法（31P-NMR）来探究运动过程中收缩肌肉内引发交感神经流出反射性刺激的细胞事件。在清醒的人类受试者中，我们对运动的前臂肌肉进行31P-NMR检测，并同时用微电极记录腓总神经中的肌肉交感神经活动（MSNA），以确定MSNA的激活是否与肌肉pH值（糖酵解指标）相关，或者与作为线粒体呼吸调节剂的无机磷酸盐（Pi）和二磷酸腺苷（ADP）的浓度（II）相关。在静态和节律性握力运动期间，静息肌肉中交感神经激活的起始与活动肌肉中细胞酸化的发展同时出现。此外，MSNA的增加与细胞内pH值的降低密切相关，但与磷酸肌酸[（PCr）]、[Pi]和[ADP]的变化无关。主要的新结论是，人类运动过程中肌肉的激活与收缩肌肉中质子的细胞内积累相关。
