Hashemian Fatemeh, Ghafouri-Fard Soudeh, Arsang-Jang Shahram, Mirzajani Sara, Fallah Hamid, Mehvari Habibabadi Jafar, Sayad Arezou, Taheri Mohammad
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Clinical Research Development Center (CRDU), Qom University of Medical Sciences, Qom, Iran.
Front Mol Biosci. 2019 Oct 23;6:113. doi: 10.3389/fmolb.2019.00113. eCollection 2019.
Long non-coding RNAs (lncRNAs) are a group of functional transcripts that are not translated to proteins. Recent investigations have underscored their role in the pathogenesis of neurodevelopmental disorders. In the current investigation, we quantified expression levels of four lncRNAs (, and ) in peripheral blood of epileptic patients and normal controls. Expression of was significantly higher in patients compared with controls (Posterior beta = 1.982, = 0.001). We detected interaction effects of gender on expression of ( = 0.012). Further analyses showed over-expression of in male patients compared with male controls ( = 0.003), in spite of similar levels of expression between female cases and female controls ( = 0.77). Expression of was higher in total patients compared with total controls (Posterior beta = 1.27, = 0.02). Such difference was only observed between male patients and male controls when dividing study participants based on their gender ( = 0.012). There was no significant difference in expression of and between patients and controls. Expression levels of all lncRNAs were correlated with each other with r values ranging from 0.61 to 0.76 ( < 0.0001). However, expressions of none of lncRNAs were correlated with age of study participants. The current data implies a putative role for two lncRNAs in the pathogenesis of epilepsy and warrants future functional studies to verify the observed association.
长链非编码RNA（lncRNAs）是一类不翻译成蛋白质的功能性转录本。最近的研究强调了它们在神经发育障碍发病机制中的作用。在当前的研究中，我们对癫痫患者和正常对照外周血中四种lncRNAs（……以及……）的表达水平进行了定量分析。与对照组相比，患者中……的表达显著更高（后验β = 1.982，P = 0.001）。我们检测到性别对……表达的交互作用（P = 0.012）。进一步分析表明，与男性对照组相比，男性患者中……过度表达（P = 0.003），尽管女性病例和女性对照组之间的表达水平相似（P = 0.77）。与总对照组相比，患者总体中……的表达更高（后验β = 1.27，P = 0.02）。当根据性别对研究参与者进行划分时，这种差异仅在男性患者和男性对照组之间观察到（P = 0.012）。患者和对照组之间……以及……的表达没有显著差异。所有lncRNAs的表达水平相互关联，r值范围为0.61至0.76（P < 0.0001）。然而，没有一种lncRNAs的表达与研究参与者的年龄相关。当前数据表明两种lncRNAs在癫痫发病机制中可能发挥作用，值得未来进行功能研究以验证所观察到的关联。
