Institute of Human Genetics, Jena University Hospital, Jena, Germany.
Urology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Acta Neurol Belg. 2024 Aug;124(4):1295-1301. doi: 10.1007/s13760-024-02513-0. Epub 2024 Apr 16.
Current studies have shown emerging roles of lncRNAs in the pathobiology of neuropathic pain and migraine.
We have chosen five lncRNAs, namely, PVT1, DSCAM-AS, MEG3, LINC-ROR, and SPRY4-IT1 for assessment of their expression in the circulation of migraineurs.
Expressions of PVT1 and MEG3 were higher in total migraineurs and both subgroups compared with controls (P < 0.0001). Meanwhile, expression of both lncRNA was higher in migraineurs with aura versus migraineurs without aura (P value < 0.0001 and = 0.01, respectively). Expression of DSCAM-AS1 was not different between any groups of patients compared with controls. Expression of LINC-ROR was elevated in total patients and patients with aura compared with controls (P value = 0.0002 and < 0.0001, respectively). It was also over-expressed in migraineurs with aura vs. migraineurs without aura (P = 0.01). Finally, expression of SPRY4-IT1 was higher in total patients and patients without aura compared with migraine-free persons (P values < 0.0001). Expressions of five mentioned lncRNAs were correlated in almost all study groups. In patients without aura, correlations were significant only for two pairs (SPRY4-IT1/PVT1 and SPRY4-IT1/DSCAM-AS1). PVT1 and MEG3 had the appropriate AUC, sensitivity and specificity values for separation of total migraineurs and both groups of patients from controls. The highest AUC value was reported for PVT1 in separation of migraineurs with aura from healthy controls (AUC = 0.98).
Cumulatively, our study shows evidence for deregulation of lncRNAs in migraineurs.
目前的研究表明，lncRNAs 在神经病理性疼痛和偏头痛的病理生物学中发挥着新的作用。
我们选择了五个 lncRNAs，即 PVT1、DSCAM-AS、MEG3、LINC-ROR 和 SPRY4-IT1，用于评估它们在偏头痛患者循环中的表达。
总偏头痛患者和两个亚组患者的 PVT1 和 MEG3 表达均高于对照组（P<0.0001）。同时，有先兆偏头痛患者和无先兆偏头痛患者的这两种 lncRNA 表达均高于无先兆偏头痛患者（P 值分别<0.0001 和=0.01）。与对照组相比，DSCAM-AS1 在任何患者组之间的表达均无差异。总患者和有先兆患者的 LINC-ROR 表达均高于对照组（P 值分别为=0.0002 和<0.0001）。它在有先兆偏头痛患者中也表现出高表达（P=0.01）。最后，总患者和无先兆偏头痛患者的 SPRY4-IT1 表达均高于无偏头痛患者（P 值均<0.0001）。在几乎所有研究组中，五种提到的 lncRNA 的表达均相关。在无先兆偏头痛患者中，仅在两对之间具有显著相关性（SPRY4-IT1/PVT1 和 SPRY4-IT1/DSCAM-AS1）。PVT1 和 MEG3 对于将总偏头痛患者和两个患者组与对照组区分开来具有适当的 AUC、敏感性和特异性值。PVT1 在将有先兆偏头痛患者与健康对照组区分开来时，报告了最高的 AUC 值（AUC=0.98）。
总之，我们的研究表明，lncRNAs 在偏头痛患者中存在失调。
