Department of Chemistry, Institute of Sciences, Gazi University, Ankara, Turkey.
Department of Chemistry, Faculty of Engineering and Natural Sciences, İstanbul Medeniyet University, Istanbul, Turkey.
Prep Biochem Biotechnol. 2020;50(3):240-245. doi: 10.1080/10826068.2019.1687518. Epub 2019 Nov 9.
A new choline oxidase (ChO) and toluidine blue O (TBO) based amperometric choline biosensor was reported in this article. An amperometric choline biosensor with immobilization of TBO (as a mediator), ChO onto polypyrrole-polyvinylsulphonate (PPy-PVS) film was accomplished on the surface of a platinum electrode. ChO was immobilized on PPy-PVS film by cross-linking with glutaraldehyde (GA). TBO was used as the mediator. Choline is oxidized to betaine and hydrogen peroxide in an oxygenated environment by ChO. Mediator reduced by reaction with hydrogen peroxide. The amperometric response was based upon the electrocatalytic properties of TBO. Optimum pH and temperature values were 7.0 and 30 °C, respectively. There was linearity between 1.0 × 10 and 2.0 × 10 M ( = 0.9805). The detection limit of the biosensor was 1.0 × 10 M and response time of the biosensor was 200 s. The storage stability and reproducibility of the biosensor were also investigated. Interfering effect of several interferants such as ascorbic acid, uric acid, alanine, dopamine, paracetamol, cysteine, and glucose on the choline biosensor was examined. The developed biosensor was tested in determinations of content in a synthetic blood sample.
本文报道了一种基于新型胆碱氧化酶（ChO）和甲苯胺蓝 O（TBO）的电流型胆碱生物传感器。通过将 TBO（作为媒介物）和 ChO 固定在聚吡咯-聚砜（PPy-PVS）膜上，在铂电极表面制备了一种电流型胆碱生物传感器。ChO 通过与戊二醛（GA）交联固定在 PPy-PVS 膜上。TBO 被用作媒介物。ChO 在含氧环境中将胆碱氧化为甜菜碱和过氧化氢。媒介物通过与过氧化氢的反应而被还原。电流响应基于 TBO 的电催化性质。最佳 pH 值和温度分别为 7.0 和 30°C。在 1.0×10 和 2.0×10 M 之间存在线性关系（ = 0.9805）。该生物传感器的检测限为 1.0×10 M，响应时间为 200 s。还研究了生物传感器的存储稳定性和重现性。考察了几种干扰物，如抗坏血酸、尿酸、丙氨酸、多巴胺、对乙酰氨基酚、半胱氨酸和葡萄糖对胆碱生物传感器的干扰影响。该开发的生物传感器已用于测定合成血样中的含量。
