School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China; State Key Laboratory of Materials-oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, China.
Int J Biol Macromol. 2020 Jul 15;155:1105-1113. doi: 10.1016/j.ijbiomac.2019.11.077. Epub 2019 Nov 9.
Corn silk polysaccharides (CSPs) were extracted from the corn silk cultivated in Jilin province, China, where is one of the golden corn belts worldwide. Three fractions (CSP-1, CSP-2 and CSP-3) were obtained by DEAE-52 cellulose and the former two fractions were further purified by Sephadex G-150 column chromatography to obtain CSP-S-1 and CSP-S-2. The molecular weights of CSP-S-1 and CSP-S-2 were calculated to be 586 kDa and 813 kDa, respectively. CSP-S-1 was composed of galactose, arabinose, xylose and rhamnose at a molar ratio of 4.16:1.00:1.01:6.32 and CSP-S-2 was composed of galactose, arabinose, glucose and rhamnose at a molar ratio of 8.71:3.58:0.169:1.00. CSP-S-2 outperformed CSP-S-1 in scavenging DPPH, ABTS and hydroxyl radicals, and significantly inhibited the proliferation of HeLa cells. IR and NMR analysis indicated that CSP-S-2 was pyranose. CSP-S-2 consisted of 1 → 4 and 1 → 6 linkages and exhibited a triple helix configuration. In summary, CSP-S-2 possesses high potential to be developed as a novel antioxidant and anti-cervical cancer agent.
玉米须多糖（CSPs）从中国吉林省种植的玉米须中提取，此地是世界黄金玉米带之一。通过 DEAE-52 纤维素获得三个级分（CSP-1、CSP-2 和 CSP-3），前两个级分进一步通过 Sephadex G-150 柱层析纯化，得到 CSP-S-1 和 CSP-S-2。CSP-S-1 和 CSP-S-2 的分子量分别计算为 586 kDa 和 813 kDa。CSP-S-1 由半乳糖、阿拉伯糖、木糖和鼠李糖组成，摩尔比为 4.16:1.00:1.01:6.32，CSP-S-2 由半乳糖、阿拉伯糖、葡萄糖和鼠李糖组成，摩尔比为 8.71:3.58:0.169:1.00。CSP-S-2 在清除 DPPH、ABTS 和羟基自由基方面优于 CSP-S-1，并显著抑制 HeLa 细胞的增殖。IR 和 NMR 分析表明 CSP-S-2 为吡喃糖。CSP-S-2 由 1→4 和 1→6 键组成，呈三螺旋构象。总之，CSP-S-2 具有开发新型抗氧化剂和抗宫颈癌药物的巨大潜力。
