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不同羧基含量的玉米须多糖通过抑制纳米草酸钙晶体的内吞作用减轻肾上皮细胞的氧化损伤

Corn Silk Polysaccharides with Different Carboxyl Contents Reduce the Oxidative Damage of Renal Epithelial Cells by Inhibiting Endocytosis of Nano-calcium Oxalate Crystals.

作者信息

Zhang Yi-Han, Li Chun-Yao, Zou Guo-Jun, Xian Jun-Yi, Zhang Quan, Yu Bang-Xian, Huang Ling-Hong, Liu Hong-Xing, Sun Xin-Yuan

机构信息

Department of Urology, Guangzhou Institute of Urology, Guangdong Key Laboratory of Urology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong 510230, China.

Department of Chemistry, Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou, Guangdong 510632, China.

出版信息

ACS Omega. 2023 Jul 14;8(29):25839-25849. doi: 10.1021/acsomega.3c01306. eCollection 2023 Jul 25.

DOI:10.1021/acsomega.3c01306
PMID:37521646
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10373179/
Abstract

OBJECTIVE

Renal epithelial cell injury and cell-crystal interaction are closely related to kidney stone formation.

METHODS

This study aims to explore the inhibition of endocytosis of nano-sized calcium oxalate monohydrate (nano-COM) crystals and the cell protection of corn silk polysaccharides (CCSPs) with different carboxyl contents (3.92, 7.75, 12.90, and 16.38%). The nano-COM crystals protected or unprotected by CCSPs were co-cultured with human renal proximal tubular epithelial cells (HK-2), and then the changes in the endocytosis of nano-COM and cell biochemical indicators were detected.

RESULTS

CCSPs could inhibit the endocytosis of nano-COM by HK-2 cells and reduce the accumulation of nano-COM in the cells. Under the protection of CCSPs, cell morphology is restored, intracellular superoxide dismutase levels are increased, lipid peroxidation product malondialdehyde release is decreased, and mitochondrial membrane potential and lysosomal integrity are increased. The release of Ca ions in the cell, the level of cell autophagy, and the rate of cell apoptosis and necrosis are also reduced. CCSPs with higher carboxyl content have better cell protection abilities.

CONCLUSION

CCSPs could inhibit the endocytosis of nano-COM crystals and reduce cell oxidative damage. CCSP3, with the highest carboxyl content, shows the best biological activity.

摘要

目的

肾上皮细胞损伤与细胞 - 晶体相互作用与肾结石形成密切相关。

方法

本研究旨在探讨不同羧基含量（3.92%、7.75%、12.90%和16.38%）的玉米须多糖（CCSPs）对纳米级一水合草酸钙（nano - COM）晶体胞吞作用的抑制及细胞保护作用。将经CCSPs保护或未保护的nano - COM晶体与人肾近端小管上皮细胞（HK - 2）共培养，然后检测nano - COM胞吞作用及细胞生化指标的变化。

结果

CCSPs可抑制HK - 2细胞对nano - COM的胞吞作用，减少nano - COM在细胞内的蓄积。在CCSPs保护下，细胞形态恢复，细胞内超氧化物歧化酶水平升高，脂质过氧化产物丙二醛释放减少，线粒体膜电位和溶酶体完整性增加。细胞内钙离子释放、细胞自噬水平、细胞凋亡和坏死率也降低。羧基含量较高的CCSPs具有更好的细胞保护能力。

结论

CCSPs可抑制nano - COM晶体的胞吞作用，减轻细胞氧化损伤。羧基含量最高的CCSP3表现出最佳的生物学活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/fb200f295507/ao3c01306_0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/9e500586791e/ao3c01306_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/24bd8d5eca10/ao3c01306_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/a9b58943100f/ao3c01306_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/1d472061c000/ao3c01306_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/e782b7feb248/ao3c01306_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/492fc8107923/ao3c01306_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/7e2a81396ccb/ao3c01306_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/de9d72160618/ao3c01306_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/3e614ab143ae/ao3c01306_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/fb200f295507/ao3c01306_0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/9e500586791e/ao3c01306_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/24bd8d5eca10/ao3c01306_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/a9b58943100f/ao3c01306_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/1d472061c000/ao3c01306_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/e782b7feb248/ao3c01306_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/492fc8107923/ao3c01306_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/7e2a81396ccb/ao3c01306_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/de9d72160618/ao3c01306_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/3e614ab143ae/ao3c01306_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cc7/10373179/fb200f295507/ao3c01306_0011.jpg

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