Faculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, Jiangsu, The People's Republic of China.
College of Horticulture and Landscape Architechture, Southwest University, Chongqing, The People's Republic of China.
PeerJ. 2022 Feb 21;10:e12922. doi: 10.7717/peerj.12922. eCollection 2022.
Laccase, as a copper-containing polyphenol oxidase, primarily functions in the process of lignin, anthocyanin biosynthesis, and various abiotic/biotic stresses. In this study, forty-eight laccase members were identified in the eggplant genome. Only forty-two laccase genes from eggplant () were anchored unevenly in 12 chromosomes, the other six were mapped on unanchored scaffolds. Phylogenetic analysis indicated that only twenty-five were divided into six different groups on the basis of groups reported in . Gene structure analysis revealed that the number of exons ranged from one to 13. Motif analysis revealed that SmLACs included six conserved motifs. In aspects of gene duplication analysis, twenty-one were collinear with genes from , tomato or rice. Cis-regulatory elements analysis indicated many may be involved in eggplant morphogenesis, flavonoid biosynthesis, diverse stresses and growth/development processes. Expression analysis further confirmed that a few s may function in vegetative and reproductive organs at different developmental stages and also in response to one or multiple stresses. This study would help to further understand and enrich the physiological function of the gene family in eggplant, and may provide high-quality genetic resources for eggplant genetics and breeding.
漆酶是一种含铜多酚氧化酶，主要功能在于木质素、花青素生物合成以及各种非生物/生物胁迫过程中。本研究在茄子基因组中鉴定了 48 个漆酶成员。茄子（）中的 42 个漆酶基因仅不均匀地锚定在 12 条染色体上，其余 6 个基因映射到未锚定的支架上。系统发育分析表明，仅根据报告的分组，只有 25 个被分为六个不同的组。基因结构分析表明，外显子的数量从 1 到 13 不等。基序分析表明 SmLACs 包括六个保守基序。在基因复制分析方面，21 个与来自拟南芥、番茄或水稻的基因具有共线性。顺式调控元件分析表明，许多可能参与茄子形态发生、类黄酮生物合成、多种胁迫和生长/发育过程。表达分析进一步证实，少数在不同发育阶段的营养器官和生殖器官中以及对一种或多种胁迫的反应中可能发挥作用。本研究有助于进一步了解和丰富茄子中基因家族的生理功能，并可能为茄子遗传和育种提供高质量的遗传资源。
