Gao Jian, Peng Hua, Chen Fabo, Luo Mao, Li Wenbo
School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Fuling, Chongqing, China.
College of Tourism and Cultural Industry, Sichuan Tourism College, Chengdu, Sichuan, China.
PeerJ. 2019 Nov 7;7:e8041. doi: 10.7717/peerj.8041. eCollection 2019.
Carmine radish produced in Chongqing is famous for containing a natural red pigment (red radish pigment). However, the anthocyanin biosynthesis transcriptome and the expression of anthocyanin biosynthesis-related genes in carmine radish have not been fully investigated. Uncovering the mechanism of anthocyanin biosynthesis in the 'Hongxin 1' carmine radish cultivar has become a dominant research topic in this field. In this study, a local carmine radish cultivar named 'Hongxin 1' containing a highly natural red pigment was used to analyze transcription factors (TFs) related to anthocyanin biosynthesis during the dynamic development of fleshy roots. Based on RNA sequencing data, a total of 1,747 TFs in 64 TF families were identified according to their DNA-binding domains. Of those, approximately 71 differentially expressed transcription factors (DETFs) were commonly detected in any one stage compared with roots in the seedling stage (SS_root). Moreover, 26 transcripts of DETFs targeted by 74 miRNAs belonging to 25 miRNA families were identified, including , , , , , , , and . Finally, eight DETF transcripts belonging to the , and families and their eight corresponding miRNAs were selected for qRT-PCR to verify their functions related to anthocyanin biosynthesis during the development of carmine radish fleshy roots. Finally, we propose a putative miRNA-target regulatory model associated with anthocyanin biosynthesis in carmine radish. Our findings suggest that sucrose synthase might act as an important regulator to modulate anthocyanin biosynthesis in carmine radish by inducing several miRNAs (miR165a-5p, miR172b, miR827a, miR166g and miR1432-5p) targeting different ERFs than candidate miRNAs in the traditional WMBW complex in biological processes.
重庆产的胭脂萝卜因含有一种天然红色素（红萝卜色素）而闻名。然而，胭脂萝卜中花青素生物合成转录组及花青素生物合成相关基因的表达尚未得到充分研究。揭示‘红心1号’胭脂萝卜品种花青素生物合成机制已成为该领域的主要研究课题。本研究中，选用了一种富含高天然红色素的本地胭脂萝卜品种‘红心1号’，来分析肉质根动态发育过程中与花青素生物合成相关的转录因子（TFs）。基于RNA测序数据，根据其DNA结合结构域鉴定出64个TF家族中的1747个TF。其中，与苗期根（SS_root）相比，在任何一个阶段通常检测到约71个差异表达转录因子（DETFs）。此外，还鉴定出了属于25个miRNA家族的74个miRNA靶向的26个DETF转录本，包括 、 、 、 、 、 、 和 。最后，选择了属于 、 和 家族的8个DETF转录本及其8个相应的miRNA进行qRT-PCR，以验证它们在胭脂萝卜肉质根发育过程中与花青素生物合成相关的功能。最后，我们提出了一个与胭脂萝卜花青素生物合成相关的假定miRNA-靶标调控模型。我们的研究结果表明，蔗糖合酶可能通过诱导几种miRNA（miR165a-5p、miR172b、miR827a、miR166g和miR1432-5p），在生物学过程中靶向不同于传统WMBW复合体中候选miRNA的不同ERF，从而作为调节胭脂萝卜花青素生物合成的重要调节因子。
