The State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200050, P. R. China.
Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Small. 2021 Oct;17(42):e2102113. doi: 10.1002/smll.202102113. Epub 2021 Sep 15.
Photodynamic therapy (PDT) has attracted tremendous attention due to its advantages such as high safety and effectiveness compared to traditional radiotherapy and chemotherapy. However, the intratumoral hypoxic microenvironment will inevitably compromise the PDT effect of the highly oxygen-dependent type II photosensitizers, implicating the urgent demand for continuous intratumoral oxygenation. Herein, biocompatible photosynthetic cyanobacteria have been modified with inorganic two-dimensional black phosphorus nanosheets (BPNSs) to be a novel bioreactor termed as Cyan@BPNSs. Upon 660 nm laser irradiation, the photosynthetic cyanobacteria generate oxygen continuously in situ through photosynthesis, followed by the photosensitization of BPNSs for activating oxygen into singlet oxygen ( O ), resulting in a large amount of O accumulation at the tumor site and the consequent strong tumor cell killing effect both in vitro and in vivo. This work provides an attractive strategy for efficient and biocompatible PDT, meanwhile extends the scope of microbiotic nanomedicine by hybridizing microorganisms with inorganic nanophotosensitizer.
光动力疗法（PDT）具有安全性高、疗效好等优点，与传统的放化疗相比，引起了人们的极大关注。然而，肿瘤内缺氧微环境不可避免地会影响高度依赖氧的 II 型光敏剂的 PDT 效果，这就迫切需要持续的肿瘤内氧合。在此，通过对生物相容性的光合蓝细菌进行无机二维黑磷纳米片（BPNSs）的修饰，得到了一种新型的生物反应器，称为 Cyan@BPNSs。在 660nm 激光照射下，通过光合作用，光合蓝细菌原位持续产生氧气，随后 BPNSs 被光敏化，将氧气激活成单线态氧（ 1 O ），导致肿瘤部位大量积累 1 O ，从而在体外和体内产生强烈的肿瘤细胞杀伤效应。这项工作为高效、生物相容性的 PDT 提供了一种有吸引力的策略，同时通过将微生物与无机纳米光敏剂杂交，扩展了微生物纳米药物学的范围。
