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超临界流体萃取法对Endl.四部提取物的成分分析及其对RAW 264.7细胞的抗炎作用

Component analysis of four-part extracts from Endl. by supercritical fluid extraction and anti-inflammatory effect on RAW 264.7cells.

作者信息

Kwak Byeong Mun, Kim Ee-Hwa, Kim Yong-Min, Kim Hee-Tack

机构信息

Department of Meridian & Acupoint, College of Korean Medicine, Semyung University, Jecheon, Korea.

Department of Oriental Medical and Herbal Cosmetic Sciences, Semyung University, Jecheon, Korea.

出版信息

J Exerc Rehabil. 2019 Oct 28;15(5):723-730. doi: 10.12965/jer.1938442.221. eCollection 2019 Oct.

Abstract

The main purpose of this study was to suggest a solution for inflammation caused by urban daily life based on supercritical extraction of Endl. (). The leaves, cone, wood, and bark of were separately extracted with supercritical extraction. Alpha-pinene, anti-inflammatory active compound of , was analyzed by electrospray ionization-mass spectrometry, gas chromatography, and gas chromatography mass spectrometry from each part of extracts. As a result of the reduction rate of nitric oxide in RAW 264.7 cells, the wood extract exhibited the highest reduction rate was about 45% among extracts from four part of . The wood extract from can be developed as a good anti-inflammatory natural raw material for natural product industry.

摘要

本研究的主要目的是基于对[植物名称未完整给出]的超临界萃取,为城市日常生活引起的炎症提出一种解决方案。分别用超临界萃取法对[植物名称未完整给出]的叶子、球果、木材和树皮进行萃取。通过电喷雾电离质谱、气相色谱和气相色谱质谱联用,对各萃取部位的α-蒎烯([植物名称未完整给出]的抗炎活性化合物)进行分析。以RAW 264.7细胞中一氧化氮的减少率为结果,木材提取物在[植物名称未完整给出]四个部位的提取物中表现出最高的减少率,约为45%。[植物名称未完整给出]的木材提取物可开发成为天然产品行业中一种优良的抗炎天然原料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cb1/6834702/49e43469f1c5/jer-15-5-723f1.jpg

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