ICAR-National Institute of Abiotic Stress Management, Malegaon, Baramati, Pune, 413115, India.
Fish Physiol Biochem. 2020 Feb;46(1):315-329. doi: 10.1007/s10695-019-00719-1. Epub 2019 Nov 15.
Global warming due to increasing temperature and contamination in aquatic environment has been found to be inducing cellular metabolic stress in fish. The present study focused on temperature and contamination in aquatic ecosystems and its alleviation/mitigation. Hence, this study was conducted to evaluate the role of zinc to improve growth performance, cellular metabolic stress, and digestive enzymes of the Pangasianodon hypophthalmus reared under lead (Pb) and high temperature. Two hundred and seventy-three fishes were distributed randomly into seven treatments, each with three replicates. Three isocaloric and isonitrogenous diets with graded levels of zinc at 0 mg/kg, 10 mg/kg, and 20 mg/kg were prepared. The Pb in treated water was maintained at the level of 1/21th of LC (4 ppm) and maintained at a temperature of 34 °C in exposure groups. The growth performance in terms of weight gain (%), protein efficiency ratio (PER), and specific growth rate (SGR) was found to be inhibited, and the feed conversion ratio (FCR) was enhanced in the Pb and high temperature-exposed group, whereas zinc supplementation has improved weight gain (%), FCR, PER, and SGR. The liver, gill, muscle, and kidney tissues of carbohydrate metabolic enzymes (LDH and MDH), protein metabolic enzymes (ALT and AST), and liver, gill, and muscle G6PDH and ATPase as well as intestinal digestives enzymes (proteases, amylase, and lipase) and intestinal ALP were significantly affected (p < 0.01) by Pb and high temperature exposure to P. hypophthalmus. We herein report the role of zinc in mitigating cellular metabolic stress in fish exposed to Pb and high temperature.
由于温度升高和水环境污染导致的全球变暖，已被发现会使鱼类产生细胞代谢应激。本研究侧重于水生生态系统中的温度和污染及其缓解/减轻。因此，本研究旨在评估锌的作用，以提高在铅（Pb）和高温下饲养的 Pangasianodon hypophthalmus 的生长性能、细胞代谢应激和消化酶。将 273 条鱼随机分配到七个处理组中，每个处理组有三个重复。制备了三种等热量和等氮的饲料，锌的水平分别为 0 mg/kg、10 mg/kg 和 20 mg/kg。处理水中的 Pb 保持在 LC（4 ppm）的 1/21 水平，并在暴露组中保持在 34°C 的温度。体重增加（%）、蛋白质效率比（PER）和特定生长率（SGR）等生长性能受到抑制，而 Pb 和高温暴露组的饲料转化率（FCR）提高，而锌补充则提高了体重增加（%）、FCR、PER 和 SGR。肝脏、鳃、肌肉和肾脏组织的碳水化合物代谢酶（LDH 和 MDH）、蛋白质代谢酶（ALT 和 AST）以及肝脏、鳃和肌肉的 G6PDH 和 ATPase 以及肠道消化酶（蛋白酶、淀粉酶和脂肪酶）和肠道碱性磷酸酶（ALP）均受到 Pb 和高温暴露对 P. hypophthalmus 的显著影响（p < 0.01）。本研究报告了锌在减轻 Pb 和高温暴露的鱼类细胞代谢应激中的作用。
