评估锰锌铁氧体纳米颗粒作为检测4T1乳腺癌细胞的新型磁共振成像造影剂。

Assessment of Manganese-Zinc Ferrite Nanoparticles as a Novel Magnetic Resonance Imaging Contrast Agent for the Detection of 4T1 Breast Cancer Cells.

作者信息

Sobhani Tayebe, Shahbazi-Gahrouei Daryoush, Rostami Mahboubeh, Zahraei Maryam, Farzadniya Amin

机构信息

Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Department of Medicinal Chemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

J Med Signals Sens. 2019 Oct 24;9(4):245-251. doi: 10.4103/jmss.JMSS_59_18. eCollection 2019 Oct-Dec.

BACKGROUND

The aim of the study was to evaluate the potential of manganese-zinc ferrite nanoparticles (MZF NPs) as a novel negative magnetic resonance imaging (MRI) contrast agents for 4T1 (mouse mammary carcinoma) and L929 (murine fibroblast) cell lines.

METHODS

MZF NPs and its suitable coating, polyethylene glycol (PEG) via covalent bonding, were investigated under condition. The cytotoxicity of MZF NPs was tested by 3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide assay after 12 and 24 h of incubation. To evaluate the potential of MZF NPs as T MRI nanocontrast agent, images were obtained from phantom containing different Fe concentrations and T relaxivity ( ) was measured. The viability of both 4T1 breast cancer and L929 murine fibroblast cell lines incubated with different Fe concentrations.

RESULTS

T-weighted MRI showed that signal intensity of 4T1 cells was lower than that of L929 as control cells. T-weighted MRI showed that signal intensity of MZF NPs enhanced with increasing concentration of NPs. The values of 1/T relaxivity ( ) for coated MZF NPs with PEG found to be 85.5 mM s which is higher than that of commercially clinical used (Sinerem) MRI contrast agent.

CONCLUSION

The results showed that MZF NPs have potential to detect breast cancer cells (4T1) and also have high contrast resolution between normal (L929) and cancerous cells (4T1) which is a suitable nanoprobe for T-weighted MR imaging contrast agents.

背景

本研究的目的是评估锰锌铁氧体纳米颗粒（MZF NPs）作为一种新型的用于4T1（小鼠乳腺癌）和L929（小鼠成纤维细胞）细胞系的阴性磁共振成像（MRI）造影剂的潜力。

方法

在特定条件下研究了MZF NPs及其通过共价键合的合适涂层聚乙二醇（PEG）。孵育12小时和24小时后，通过3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法检测MZF NPs的细胞毒性。为了评估MZF NPs作为T MRI纳米造影剂的潜力，从含有不同铁浓度的模型中获取图像并测量T弛豫率（）。用不同铁浓度孵育4T1乳腺癌和L929小鼠成纤维细胞系，检测其活力。