Convergence of Smart Sensors Institute, Dong-Eui University, Busan, Republic of Korea.
Convergence of Smart Sensors Institute, Dong-Eui University, Busan, Republic of Korea; Micro and Nano Engineering Laboratory, Department of Electronic Engineering, Sogang University, Seoul, Republic of Korea.
J Colloid Interface Sci. 2019 Apr 1;541:192-203. doi: 10.1016/j.jcis.2019.01.063. Epub 2019 Jan 17.
This article outlines the synthesis of gadolinium (Gd)-doped manganese zinc ferrite magnetic nanoparticles (MNPs) as potential magnetic carriers for magnetic fluid hyperthermia (MFH). MNPs with high specific loss power (SLP; 146 W/g) have been developed and used for an in vitro hyperthermia study. The treatment of MFH is fruitful if there is an adequate number of MNPs in tumor cells with the highest SLP to rapidly generate heat while minimizing thermal injury to surrounding healthy tissue. X-ray diffraction patterns of the studied particles confirm the formation of a cubic spinel structure. Field emission scanning electron micrographs showed homogeneous distributions of particles with some agglomerates with a granular appearance. Transmission electron microscopy analysis showed the presence of agglomerated spherical particles at the surface. The substitution of Gd resulted in superparamagnetism at room temperature as confirmed by vibrating sample magnetometer analysis. The estimated saturation magnetization reduced from 48.6 to 28.2 emu/g with an increase in Gd concentration. However, the coercivity increased from 1093 Oe to 1597 Oe. Field cooled and zero field cooled measurements showed Curie temperatures from 315 to 326 K, as required for MFH applications. Cell viability measurements indicated that the MNPs are nontoxic to A549 cells for the studied concentrations of particle fraction and a contact time of up to 24 h. The interaction of the MNPs with A549 cells was highlighted from an image captured by an inverted microscope. In order to treat cancer in vivo, an in vitro hyperthermia study has initially been carried out with A549 cells.
本文概述了镝（Gd）掺杂锰锌铁氧体磁性纳米粒子（MNPs）的合成，作为用于磁流体热疗（MFH）的潜在磁性载体。已经开发出具有高比损耗功率（SLP；146 W/g）的 MNPs，并将其用于体外热疗研究。如果肿瘤细胞中有足够数量的 MNPs 具有最高的 SLP，能够快速产生热量，同时最大限度地减少对周围健康组织的热损伤，那么 MFH 的治疗效果将是富有成效的。研究颗粒的 X 射线衍射图谱证实了立方尖晶石结构的形成。场发射扫描电子显微镜照片显示颗粒分布均匀，有些颗粒团聚呈粒状。透射电子显微镜分析表明，表面存在团聚的球形颗粒。通过振动样品磁强计分析证实，Gd 的取代导致室温下的超顺磁性。估计的饱和磁化强度从 48.6 降低到 28.2 emu/g，随着 Gd 浓度的增加。然而，矫顽力从 1093 Oe 增加到 1597 Oe。场冷却和零场冷却测量显示居里温度从 315 到 326 K，这是 MFH 应用所需的。细胞活力测量表明，在所研究的粒子分数浓度和长达 24 h 的接触时间下，MNPs 对 A549 细胞是无毒的。通过倒置显微镜拍摄的图像突出显示了 MNPs 与 A549 细胞的相互作用。为了在体内治疗癌症，首先在 A549 细胞上进行了体外热疗研究。
