TORC1 调控液泡膜上的 ESCRT-0 复合物形成和酵母中的微自噬诱导。
TORC1 regulates ESCRT-0 complex formation on the vacuolar membrane and microautophagy induction in yeast.
机构信息
Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan; Department of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
出版信息
Biochem Biophys Res Commun. 2020 Jan 29;522(1):88-94. doi: 10.1016/j.bbrc.2019.11.064. Epub 2019 Nov 15.
Microautophagy is promoted after nutrient starvation and inactivation of target of rapamycin complex 1 (TORC1) kinase. Invagination of vacuolar membranes by endosomal sorting complex required for transport (ESCRT) is required for microautophagy. Vps27, a subunit of ESCRT-0, is recruited onto vacuolar membranes via dephosphorylation after TORC1 inactivation. Here, we showed that Hse1, another ESCRT-0 subunit, is also recruited onto vacuolar membranes after TORC1 inactivation, promoting formation of ESCRT-0 complex on vacuolar membranes. Hse1 recruitment was dependent on Vps27, whereas Vps27 recruitment was independent of Hse1. Not only Vps27 but also Hse1 was required for ESCRT-III recruitment onto vacuolar membranes and microautophagy induction after TORC1 inactivation. This study revealed that ESCRT-0 (Vps27-Hse1) complex formation on vacuolar membranes is important for microautophagy inactivation after TORC1 inactivation.
微自噬在营养饥饿和雷帕霉素靶蛋白复合物 1 (TORC1) 激酶失活后被促进。内体分选复合物所需的运输 (ESCRT) 的液泡膜内陷是微自噬所必需的。Vps27 是 ESCRT-0 的一个亚基，在 TORC1 失活后通过去磷酸化被招募到液泡膜上。在这里，我们表明另一个 ESCRT-0 亚基 Hse1 在 TORC1 失活后也被招募到液泡膜上，促进 ESCRT-0 复合物在液泡膜上的形成。Hse1 的募集依赖于 Vps27，而 Vps27 的募集不依赖于 Hse1。不仅 Vps27，而且 Hse1 都需要招募到液泡膜上，才能在 TORC1 失活后招募 ESCRT-III 并诱导微自噬。本研究揭示了液泡膜上 ESCRT-0 (Vps27-Hse1) 复合物的形成对于 TORC1 失活后微自噬的失活很重要。
Zotero 插件