State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, 510642, China.
Environ Sci Pollut Res Int. 2019 Dec;26(36):36680-36687. doi: 10.1007/s11356-019-06671-0. Epub 2019 Nov 18.
Transporters play an important role in the uptake and redistribution of agrochemicals to the site of insect feeding. The product of the Arabidopsis thaliana gene AtAAP1 substantially contributes to inorganic nitrogen acquisition under ecologically relevant amino acid concentrations. Here, the transporter ability of AtAAP1 to a chlorantraniliprole-alanine conjugate (CAP-Ala-1) was tested both in planta and in vitro. Thirty-day-old and 15-day-old plants overexpressing AtAAP1 increased the uptake of CAP-Ala-1 into the roots, whereas AtAAP1 deficiency did not completely block the uptake of CAP-Ala-1. An uptake experiment carried out in Xenopus laevis oocytes expressing AtAAP1 showed that CAP-Ala-1 interacted with AtAAP1. Although little native AtAAP1 transcription was present in the leaves, constitutive expression of AtAAP1 in plants significantly increased the ability of the leaf mesophyll protoplasts to take up CAP-Ala-1. The observations supported the possibility of exploiting AtAAP1 as a component of a novel delivery and redistribution system for amino acid-based pesticide conjugates.
转运蛋白在农药摄取和向昆虫取食部位再分布中起着重要作用。拟南芥基因 AtAAP1 的产物在与生态相关的氨基酸浓度下，对无机氮的获取有很大贡献。在此，通过体内和体外实验测试了 AtAAP1 对氯虫苯甲酰胺-丙氨酸轭合物（CAP-Ala-1）的转运能力。过表达 AtAAP1 的 30 日龄和 15 日龄植株增加了 CAP-Ala-1 向根部的摄取，而 AtAAP1 缺乏并不能完全阻止 CAP-Ala-1 的摄取。在表达 AtAAP1 的非洲爪蟾卵母细胞中进行的摄取实验表明，CAP-Ala-1 与 AtAAP1 相互作用。尽管叶片中存在的天然 AtAAP1 转录本很少，但植物中 AtAAP1 的组成型表达显著提高了叶片叶肉原生质体摄取 CAP-Ala-1 的能力。这些观察结果支持了利用 AtAAP1 作为基于氨基酸的农药轭合物新型传递和再分配系统的组成部分的可能性。
