Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
Food Funct. 2019 Dec 11;10(12):8137-8148. doi: 10.1039/c9fo01890e.
High fructose (HF) diet-induced liver steatosis is associated with intestinal microbiota dysbiosis. The aim of this study was to assess the modulatory effects of Artemisia sphaerocephala Krash seed polysaccharide (ASKP) on fatty acid metabolism and intestinal microbiota in mice fed with HF water. Administration of HF-fed mice with ASKP prevented fat accumulation and blunted metabolic inflammation and endotoxaemia. ASKP-treated mice displayed improved glucose tolerance and fully exhibited protection against hepatic steatosis. Besides, ASKP was effective in improving the changes in the composition of liver fatty acids via modulating hepatic SREBP-1c, SCD-1, ACC and FAS expressions. 16S rRNA gene sequencing showed that ASKP treatment modified the gut microbial species at the phylum level with a decrease of Firmicutes and a slight increase of Bacteroidetes (p > 0.05). Interestingly, ASKP markedly increased the proportion of the mucin-degrading bacterium Akkermansia at the genus level in HF-fed mice. These findings support the beneficial metabolic effects of ASKP through amelioration of the HF-induced features of liver steatosis, which is associated with health maintenance of the intestinal microecosystem.
高果糖（HF）饮食诱导的肝脂肪变性与肠道微生物失调有关。本研究旨在评估沙蒿多糖（ASKP）对 HF 水喂养小鼠脂肪酸代谢和肠道微生物的调节作用。给予 HF 喂养的小鼠 ASKP 可防止脂肪堆积，并减轻代谢性炎症和内毒素血症。ASKP 治疗的小鼠表现出改善的葡萄糖耐量，并完全防止肝脂肪变性。此外，ASKP 通过调节肝 SREBP-1c、SCD-1、ACC 和 FAS 的表达，有效改善肝脂肪酸组成的变化。16S rRNA 基因测序显示，ASKP 处理在门水平上改变了肠道微生物种类，厚壁菌门减少，拟杆菌门略有增加（p > 0.05）。有趣的是，ASKP 显著增加了 HF 喂养小鼠中粘蛋白降解菌 Akkermansia 的比例。这些发现支持 ASKP 的有益代谢作用，通过改善 HF 诱导的肝脂肪变性特征，与肠道微生态系统的健康维护有关。
