A Simple Red Emitting "Turn-On" Optical Relay Detector for Al and CN. Application in the Real Sample and RAW264.7 Cell Imaging.

A quantitative and qualitative fluorogenic molecular probe (R) has been designed and synthesized using Rhodamine-B hydrazide and 3,5-dibromo salicylaldehyde. The probe R has been applied for detection of ionic species and probe R turned out to be a prominent distinguisher among divalent and trivalent cations Al, Cr and Fe via the fluorometric response in acetonitrile medium. Colorimetric changes were observed for trivalent cations and Cu. Among all trivalent cations, Al is possessing relay recognition for CN even in the presence of other interfering anions with optimum precision. The association constant and the detection limit for R-Al are 4.5 × 10 M and 17.9 nM respectively. On the other hand, R + Al+CN exhibits an association constant and detection limit of 5.77 × 10 M and 4.6 nM respectively. The stoichiometry of Al binding with R is found to be 1:1. Quantum efficiency of probe R, R + Al and R + Al+CN are found to be 0.2, 0.88 and 0.04 respectively. The receptor showed excellent real-time applicability for commercially available antiperspirant and apple seeds extract. The efficiency of the receptor further extended for fluorescent imaging of Al and CN in RAW264.7 cells.