Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
Department of Veterinary Laboratory Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
Adipocyte. 2019 Dec;8(1):357-361. doi: 10.1080/21623945.2019.1693746.
Beige adipocytes, which consume energy mainly in an uncoupling protein 1 (UCP1)-dependent manner, are risen in white adipose tissue (WAT) depots. Since beige adipocyte development is gaining attention as a potential strategy for conquering obesity, worldwide researchers are making efforts to study its biological aspects. However, assessing UCP1 protein levels in beige adipocytes is challenging because of the high level of lipid contaminants in WAT. This study showed that an acetone precipitation method had advantages over conventional methods for eliminating lipid contaminants, achieving clear Western blot bands for WAT proteins, especially UCP1. Our results suggest that the acetone precipitation cleaning method could be useful for the clear analysis and precise evaluation of WAT proteins.
米色脂肪细胞主要通过解偶联蛋白 1（UCP1）依赖的方式消耗能量，在白色脂肪组织（WAT）中增加。由于米色脂肪细胞的发育作为克服肥胖的潜在策略受到关注，全世界的研究人员都在努力研究其生物学方面。然而，由于 WAT 中的脂质污染物水平很高，评估米色脂肪细胞中的 UCP1 蛋白水平具有挑战性。本研究表明，与传统方法相比，丙酮沉淀法在去除脂质污染物方面具有优势，可获得 WAT 蛋白，特别是 UCP1 的清晰 Western blot 条带。我们的结果表明，丙酮沉淀清洗方法可用于 WAT 蛋白的清晰分析和精确评估。
