Lima-Barbero José Francisco, Contreras Marinela, Mateos-Hernández Lourdes, Mata-Lorenzo Francisco Manuel, Triguero-Ocaña Roxana, Sparagano Olivier, Finn Robert D, Strube Christina, Price Daniel R G, Nunn Francesca, Bartley Kathryn, Höfle Ursula, Boadella Mariana, Nisbet Alasdair J, Fuente José de la, Villar Margarita
SaBio. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071, Ciudad Real, Spain.
Sabiotec, S.A. Ed. Polivalente UCLM. Camino de Moledores. 13005. Ciudad Real, Spain.
Vaccines (Basel). 2019 Nov 20;7(4):190. doi: 10.3390/vaccines7040190.
The poultry red mite (PRM), , is a hematophagous ectoparasite considered as the major pest in the egg-laying industry. Its pesticide-based control is only partially successful and requires the development of new control interventions such as vaccines. In this study, we follow a vaccinology approach to identify PRM candidate protective antigens. Based on proteomic data from fed and unfed nymph and adult mites, we selected a novel PRM protein, calumenin (Deg-CALU), which is tested as a vaccine candidate on an on-hen trial. Subolesin (Rhm-SUB) was chosen as a positive control. Deg-CALU and Rhm-SUB reduced the mite oviposition by 35 and 44%, respectively. These results support Deg-CALU and Rhm-SUB as candidate protective antigens for the PRM control.
家禽红螨（PRM）是一种吸血性体外寄生虫，被认为是蛋鸡养殖行业的主要害虫。基于杀虫剂的防治方法仅取得了部分成功，因此需要开发新的防治手段，如疫苗。在本研究中，我们采用疫苗学方法来鉴定PRM候选保护性抗原。基于饱血和未饱血若螨及成螨的蛋白质组数据，我们选择了一种新型PRM蛋白——钙网蛋白（Deg-CALU），并在母鸡试验中作为疫苗候选物进行测试。选择亚精蛋白（Rhm-SUB）作为阳性对照。Deg-CALU和Rhm-SUB分别使螨虫产卵量减少了35%和44%。这些结果支持Deg-CALU和Rhm-SUB作为控制PRM的候选保护性抗原。
