一种用于鉴定和表征控制家禽红螨感染候选保护性抗原的疫苗学方法。
A vaccinology Approach to the Identification and Characterization of Candidate Protective Antigens for the Control of Poultry Red Mite Infestations.
作者信息
Lima-Barbero José Francisco, Contreras Marinela, Mateos-Hernández Lourdes, Mata-Lorenzo Francisco Manuel, Triguero-Ocaña Roxana, Sparagano Olivier, Finn Robert D, Strube Christina, Price Daniel R G, Nunn Francesca, Bartley Kathryn, Höfle Ursula, Boadella Mariana, Nisbet Alasdair J, Fuente José de la, Villar Margarita
机构信息
SaBio. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071, Ciudad Real, Spain.
Sabiotec, S.A. Ed. Polivalente UCLM. Camino de Moledores. 13005. Ciudad Real, Spain.
出版信息
Vaccines (Basel). 2019 Nov 20;7(4):190. doi: 10.3390/vaccines7040190.
The poultry red mite (PRM), , is a hematophagous ectoparasite considered as the major pest in the egg-laying industry. Its pesticide-based control is only partially successful and requires the development of new control interventions such as vaccines. In this study, we follow a vaccinology approach to identify PRM candidate protective antigens. Based on proteomic data from fed and unfed nymph and adult mites, we selected a novel PRM protein, calumenin (Deg-CALU), which is tested as a vaccine candidate on an on-hen trial. Subolesin (Rhm-SUB) was chosen as a positive control. Deg-CALU and Rhm-SUB reduced the mite oviposition by 35 and 44%, respectively. These results support Deg-CALU and Rhm-SUB as candidate protective antigens for the PRM control.
家禽红螨（PRM）是一种吸血性体外寄生虫，被认为是蛋鸡养殖行业的主要害虫。基于杀虫剂的防治方法仅取得了部分成功，因此需要开发新的防治手段，如疫苗。在本研究中，我们采用疫苗学方法来鉴定PRM候选保护性抗原。基于饱血和未饱血若螨及成螨的蛋白质组数据，我们选择了一种新型PRM蛋白——钙网蛋白（Deg-CALU），并在母鸡试验中作为疫苗候选物进行测试。选择亚精蛋白（Rhm-SUB）作为阳性对照。Deg-CALU和Rhm-SUB分别使螨虫产卵量减少了35%和44%。这些结果支持Deg-CALU和Rhm-SUB作为控制PRM的候选保护性抗原。
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