Liu Gaofeng, Khan Nadeem, Ma Xiaoqing, Hou Xilin
State Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of the P.R. China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of the P.R. China, Nanjing 210095, China.
Ottawa Research and Development Center, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada.
Plants (Basel). 2019 Nov 20;8(12):526. doi: 10.3390/plants8120526.
Histone modifications, such as methylation and demethylation, are vital for regulating chromatin structure, thus affecting its expression patterns The objective of this study is to understand the phylogenetic relationships, genomic organization, diversification of motif modules, gene duplications, co-regulatory network analysis, and expression dynamics of histone lysine methyltransferases and histone demethylase in . We identified 60 (HKMTases), 53 , and 4 (HDMases) genes in . The domain composition analysis subcategorized them into seven and nine subgroups, respectively. Duplication analysis for paralogous pairs of SET and JmjC (eight and nine pairs, respectively) exhibited variation. Interestingly, three pairs of SET exhibited > 1.00 values, signifying positive selection, whereas the remaining underwent purifying selection with values less than 1.00. Furthermore, RT-PCR validation analysis and RNA-sequence data acquired on six different tissues (i.e., leaf, stem, callus, silique, flower, and root) revealed dynamic expression patterns. This comprehensive study on the abundance, classification, co-regulatory network analysis, gene duplication, and responses to heat and cold stress of SET and JmjC provides insights into the structure and diversification of these family members in . This study will be helpful to reveal functions of these putative and genes in .
组蛋白修饰，如甲基化和去甲基化，对于调节染色质结构至关重要，从而影响其表达模式。本研究的目的是了解组蛋白赖氨酸甲基转移酶和组蛋白去甲基酶在……中的系统发育关系、基因组组织、基序模块的多样化、基因复制、共调控网络分析以及表达动态。我们在……中鉴定出60个组蛋白赖氨酸甲基转移酶（HKMTases）、53个……和4个组蛋白去甲基酶（HDMases）基因。结构域组成分析将它们分别细分为七个和九个亚组。对SET和JmjC的旁系同源对（分别为八对和九对）进行的复制分析显示出差异。有趣的是，三对SET的ω值大于1.00，表明存在正选择，而其余的则经历了ω值小于1.00的纯化选择。此外，对六个不同组织（即叶、茎、愈伤组织、角果、花和根）进行的RT-PCR验证分析和RNA序列数据揭示了动态表达模式。这项关于SET和JmjC的丰度、分类、共调控网络分析、基因复制以及对热和冷胁迫的响应的综合研究，为这些家族成员在……中的结构和多样化提供了见解。这项研究将有助于揭示这些假定的……和……基因在……中的功能。
