Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Int J Biol Macromol. 2020 Jun 1;152:1265-1273. doi: 10.1016/j.ijbiomac.2019.10.223. Epub 2019 Nov 20.
In this study, the structure characteristic of the Hericium erinaceus polysaccharide (HEP) was investigated using Fourier transformed infrared spectrometry (FT-IR), gas chromatography-mass spectrometry (GC-MS), gel permeation chromatography (GPC), methylation and nuclear magnetic resonance (NMR). The results showed that HEP, with a molecular weight of 43 KDa, was mainly composed of glucose and rhamnose. The linkages of the sugar residues of HEP were → 6) β-d-Glcp-(1 → and → 2) -α-l-Rhap-(1 → residue at the end of the branches. The Fusarium toxin deoxynivalenol (DON)-induced cellular injury model for IPEC-J2 cells was established and used to investigate the protective effects of HEP against the oxidative stress. The results suggest that HEP could significantly protect IPEC-J2 cells from DON-induced oxidative stress, inhibit DON-induced apoptosis and reduce the production of reactive oxygen species (ROS). Overall, this study suggested that HEP could be explored as potential antioxidant agents for DON-induced intestinal mucosa injury.
在这项研究中，使用傅里叶变换红外光谱（FT-IR）、气相色谱-质谱（GC-MS）、凝胶渗透色谱（GPC）、甲基化和核磁共振（NMR）研究了猴头菇多糖（HEP）的结构特征。结果表明，HEP 的分子量为 43 kDa，主要由葡萄糖和鼠李糖组成。HEP 糖残基的键合方式为→6）β-d-Glcp-（1→和→2）-α-l-Rhap-（1→分支末端的残基。建立了镰刀菌毒素脱氧雪腐镰刀菌烯醇（DON）诱导的 IPEC-J2 细胞损伤模型，用于研究 HEP 对氧化应激的保护作用。结果表明，HEP 能显著保护 IPEC-J2 细胞免受 DON 诱导的氧化应激，抑制 DON 诱导的细胞凋亡，减少活性氧（ROS）的产生。总的来说，这项研究表明 HEP 可以作为一种潜在的抗氧化剂，用于治疗 DON 诱导的肠道黏膜损伤。
